| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Pregnancy |
Department of Obstetrics and Gynecology, Evanston Northwestern Healthcare, Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60201
Toll-like receptor 4 (TLR-4) is a critical mediator of the cellular response to lipopolysaccharide. Our purpose was to examine the role of TLR-4 in parturition and in the regulation of expression of prostaglandin synthase (cyclooxygenase [COX]-1 and COX-2) and 15-hydroxyprostaglandin dehydrogenase (PGDH) following exposure to heat-killed Escherichia coli (HKE) in pregnant mice. Inbred TLR-4-mutant C3H/HeJ mice and inbred normal C3HeB/FeJ mice on Day 14.5 of a 19- to 20-day gestation received intrauterine injection of either HKE or sterile vehicle (PBS). Preterm or term delivery was recorded for these animals. Tissues (myometrium, decidual caps, placentas, fetal membranes, and fetuses) were collected after injection of sterile vehicle or 5 x 109 HKE bacteria (n = 5 mice per strain per treatment per time point). The COX-1, COX-2, and PGDH gene expression was determined by semiquantitative reverse transcription-polymerase chain reaction. We found that 5 x 109 HKE induced preterm delivery in 100% of TLR-4-normal mice but in 0% of TLR-4-mutant mice. The HKE exposure up-regulated expression of COX-2, but not of COX-1, in maternal tissues in both mouse strains. The prostaglandin-catabolizing enzyme PGDH was down-regulated in myometrium, fetal membranes, and fetuses in control mice, but no change was observed in TLR-4-mutant mice after HKE treatment. These results demonstrate that a functional TLR-4 is essential for HKE-induced preterm labor and PGDH down-regulation but is not essential for HKE-induced COX-2 gene up-regulation. The TLR-4 may mediate bacterially induced preterm labor via regulation of prostaglandin degradation rather than prostaglandin synthesis.
2 Correspondence: Emmet Hirsch, Department of Obstetrics and Gynecology, Evanston Northwestern Healthcare, 2650 Ridge Avenue, Evanston, IL 60201. FAX: 847 733 5083; e-hirsch{at}northwestern.edu
This article has been cited by other articles:
|
|
 |
|
  A. W Horne, S. J Stock, and A. E King Innate immunity and disorders of the female reproductive tract Reproduction, June 1, 2008; 135(6): 739 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Koga and G. Mor Review Article: Expression and Function of Toll-Like Receptors at the Maternal--Fetal Interface Reproductive Sciences, March 1, 2008; 15(3): 231 - 242. [Abstract] [PDF]
|
|
|
|
|
|
V. M. Abrahams Antagonizing Toll-like Receptors to Prevent Preterm Labor Reproductive Sciences, February 1, 2008; 15(2): 108 - 109. [PDF]
|
|
|
|
 |
|
 J. D. Roizen, M. Asada, M. Tong, H.-H. Tai, and L. J. Muglia Preterm Birth without Progesterone Withdrawal in 15-Hydroxyprostaglandin Dehydrogenase Hypomorphic Mice Mol. Endocrinol., January 1, 2008; 22(1): 105 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ilievski, S.-J. Lu, and E. Hirsch Activation of Toll-like Receptors 2 or 3 and Preterm Delivery in the Mouse Reproductive Sciences, May 1, 2007; 14(4): 315 - 320. [Abstract] [PDF]
|
|
|
|
 |
|
 K. N. Evans, L. Nguyen, J. Chan, B. A. Innes, J. N. Bulmer, M. D. Kilby, and M. Hewison Effects of 25-Hydroxyvitamin D3 and 1,25-Dihydroxyvitamin D3 on Cytokine Production by Human Decidual Cells Biol Reprod, December 1, 2006; 75(6): 816 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, H. Hagberg, C. Mallard, C. Zhu, M. Hedtjarn, C.-F. Tiger, K. Eriksson, A. Rosen, and B. Jacobsson Disruption of Interleukin-18, but Not Interleukin-1, Increases Vulnerability to Preterm Delivery and Fetal Mortality after Intrauterine Inflammation Am. J. Pathol., September 1, 2006; 169(3): 967 - 976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Breuiller-Fouche and G. Germain Gene and protein expression in the myometrium in pregnancy and labor. Reproduction, May 1, 2006; 131(5): 837 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhao, D. Koon, and K. E Bethin Identification of transcription factors at the site of implantation in the later stages of murine pregnancy. Reproduction, March 1, 2006; 131(3): 561 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Roizen and L. J. Muglia Understanding the Timing of Birth: The Continuing Challenge to Prevent Prematurity NeoReviews, March 1, 2006; 7(3): e151 - e159. [Full Text] [PDF]
|
|
|
|
|
|
T. M Lindstrom and P. R Bennett The role of nuclear factor kappa B in human labour Reproduction, November 1, 2005; 130(5): 569 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Romero, O. Erez, and J. Espinoza Intrauterine Infection, Preterm Labor, and Cytokines Reproductive Sciences, October 1, 2005; 12(7): 463 - 465. [PDF]
|
|
|
|
|
|
K. Yoshimura and E. Hirsch Effect of Stimulation and Antagonism of Interleukin-1 Signaling on Preterm Delivery in Mice Reproductive Sciences, October 1, 2005; 12(7): 533 - 538. [Abstract] [PDF]
|
|
|
|
|
|
E. Hirsch and H. Wang The Molecular Pathophysiology of Bacterially Induced Preterm Labor: Insights From the Murine Model Reproductive Sciences, April 1, 2005; 12(3): 145 - 155. [Abstract] [PDF]
|
|
|
|
 |
|
 J. C. Condon, P. Jeyasuria, J. M. Faust, and C. R. Mendelson Surfactant protein secreted by the maturing mouse fetal lung acts as a hormone that signals the initiation of parturition PNAS, April 6, 2004; 101(14): 4978 - 4983. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
