HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 69/6/2092    most recent biolreprod.103.020081v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoshioka, K. Articles by Rodriguez-Martinez, H. Search for Related Content PubMed PubMed Citation Articles by Yoshioka, K. Articles by Rodriguez-Martinez, H. Agricola Articles by Yoshioka, K. Articles by Rodriguez-Martinez, H.

Production of Piglets Derived from In Vitro-Produced Blastocysts Fertilized and Cultured in Chemically Defined Media: Effects of Theophylline, Adenosine, and Cysteine During In Vitro Fertilization¹

National Institute of Animal Health,³ Tsukuba, Ibaraki 305-0856, Japan Department of Obstetrics and Gynaecology,⁴ Swedish University of Agricultural Sciences, Uppsala 750 07, Sweden Azabu University,⁵ Sagamihara, Kanagawa 229-8501, Japan National Institute of Agrobiological Sciences,⁶ Tsukuba, Ibaraki 305-8602, Japan

To further develop defined conditions for in vitro fertilization (IVF) and in vitro culture (IVC) of in vitro-matured porcine oocytes, we evaluated the effects of theophylline, adenosine, and cysteine in a chemically defined medium during IVF. Viability to full term of in vitro-produced blastocysts after IVF and IVC in chemically defined medium was also investigated by embryo transfer to recipients. A chemically defined medium, porcine gamate medium (PGM), was modified from porcine zygote medium (PZM-4), which was previously established. PGM was used as a basal medium for IVF and PZM-4 was for the culture of presumptive zygotes. Addition of 2.5 mM theophylline to PGM significantly increased the percentage of male pronuclear formation compared with controls (no addition). Addition of 1 µM adenosine to PGM supplemented either with or without 2.5 mM theophylline significantly reduced the number of penetrated spermatozoa compared with controls (no addition of adenosine). Supplementation with 0.2 µM cysteine in PGM containing both 2.5 mM theophylline and 1 µM adenosine further increased the percentage of development to the blastocyst stage, compared with no supplementation of cysteine, but there was no difference in fertilization parameters, such as monospermy and pronuclear formation, regardless of presence or absence of theophylline and adenosine. When Day 5 blastocysts were transferred into four recipients (20–25 blastocysts per recipient), all recipients became pregnant and farrowed a total of 21 live piglets. The present results clearly demonstrate that porcine blastocysts can be produced by IVF and IVC in chemically defined media and that they can develop to full term after embryo transfer.

¹ This work was supported by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan and from the Swedish Foundation for International Cooperation in Research and Higher Education (STINT; SLU-Japan Programme on Reproductive Biotechnology), Sweden.

² Correspondence: Koji Yoshioka, Theriogenology Section, Department of Production Diseases, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. FAX: 81 29 838 7880; kojiyos{at}affrc.go.jp

This article has been cited by other articles:

				H. Nagashima, K. Hiruma, H. Saito, R. Tomii, S. Ueno, N. Nakayama, H. Matsunari, and M. Kurome Production of Live Piglets Following Cryopreservation of Embryos Derived from In Vitro-Matured Oocytes Biol Reprod, May 1, 2007; 76(5): 900 - 905. [Abstract] [Full Text] [PDF]