Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by STERN, S.
Right arrow Articles by KENNEDY, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by STERN, S.
Right arrow Articles by KENNEDY, J. F.
Agricola
Right arrow Articles by STERN, S.
Right arrow Articles by KENNEDY, J. F.

Biology of Reproduction, Vol 7, 341-346, Copyright © 1972 by Society for the Study of Reproduction

Incorporation of Amino Acids During Maturation In Vitro by the Mouse Oocyte: Effect of Puromycin on Protein Synthesis

SAMUEL STERN 1, ADEL RAYYIS 1, , and JOSEPH F. KENNEDY 1

1 Departments of Population Dynamics and Gynecology and Obstetrics, The Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland


Mouse oocytes cultured in vitro in the presence of [U-14C] valine mature at apparently a normal rate. The amino acid is utilized by the eggs in synthesizing proteins. The amount of TCA insoluble material was decreased when 1 µg/ml puromycin was added to the culture medium. Puromycin also inhibited the normal nuclear progression of the oocytes. Aspartate does not enter the cell as readily as valine.

Submitted on July 28, 1971






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the Society for the Study of Reproduction.