Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print September 17, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.017335
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
70/1/139    most recent
biolreprod.103.017335v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kohoutek, J.
Right arrow Articles by Hampl, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kohoutek, J.
Right arrow Articles by Hampl, A.
Agricola
Right arrow Articles by Kohoutek, J.
Right arrow Articles by Hampl, A.
BIOLOGY OF REPRODUCTION 70, 139–145 (2004)
DOI: 10.1095/biolreprod.103.017335
© 2004 by the Society for the Study of Reproduction, Inc.

Gamete Biology

Temporal Distribution of CDK4, CDK6, D-Type Cyclins, and p27 in Developing Mouse Oocytes1

J. Kohoutek, P. Dvorák, and A. Hampl2

Laboratory of Molecular Embryology,3 Mendel University Brno, 613 00 Brno, Czech Republic Department of Molecular Embryology,4 Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic Centre for Cell Therapy and Tissue Repair,5 Charles University, 150 18 Prague, Czech Republic

Various molecular interactions not operating in other cell types are most likely required for mammalian oocytes to develop into fully competent eggs. This study seeks to initiate analyses of the potential oocyte-specific functions of regulators of G1/S progression—CDK4, CDK6, D-type cyclins, and p27—by first determining their expression patterns in growing and maturing mouse oocytes and in mouse embryos early after fertilization. Western blot and immunofluorescence analyses on isolated oocytes were employed to evaluate both their levels and their localization. The data show that 1) mouse oocytes contain significant amounts of all studied regulators; 2) their amounts and localization undergo dramatic changes as the oocytes grow, meiotically mature, and transit into embryogenesis; and 3) some regulators (CDK4, CDK6, cyclin D2, and p27) appear in unusual, most likely posttranslationally modified, forms. These data distinguish G1/S regulators as the potential players in molecular processes that are important for oocytes to function normally.

1 Supported in part by the Ministry of Education, Youth, and Sports of the Czech Republic (MSM 432100001, LN 00A065); by the Academy of Sciences of the Czech Republic (AV 0Z5039906); and by the Grant Agency of the Czech Republic (GA 204/01/0905).

2 Correspondence: Ales Hampl, Laboratory of Molecular Embryology, Mendel University Brno, Zemedelská 1, 613 00 Brno, Czech Republic. FAX: 420 5 4513 3298; hampl{at}mendelu.cz






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for the Study of Reproduction.