Biol Reprod
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BOR - Papers in Press, published online ahead of print September 17, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.018820
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BIOLOGY OF REPRODUCTION 70, 204–213 (2004)
DOI: 10.1095/biolreprod.103.018820
© 2004 by the Society for the Study of Reproduction, Inc.

Testis

Goliath, a Ring-H2 Mitochondrial Protein, Regulated by Luteinizing Hormone/Human Chorionic Gonadotropin in Rat Leydig Cells1

A. Guais, B. Solhonne, N. Melaine, G. Guellaën, and F. Bulle2

Unité INSERM 581,3 Hôpital Henri Mondor, 94010 Créteil, France GERM-INSERM U.435,4 Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, France

We have cloned the rat homologue of the ring-H2 protein Goliath involved in Drosophila development. The rat Goliath mRNA (1.85 kb) was translated as a major ubiquitous protein species of 28-kDa and three larger isoforms (50, 46, and 36 kDa) expressed mainly in liver, lung, stomach, heart, and thymus and barely detectable in other tissues (kidney, skeletal muscle, brain, testis, intestine, and spleen). By immunohistochemistry on rat testis sections, we localized the protein in interstitial tissue and seminiferous tubules. In tubules, Goliath was expressed mainly in postmeiotic germ cells and to a much lesser extent in Sertoli cells. In the interstitium, Goliath was exclusively present in Leydig cells. Using a series of immunolabeling, cellular fractionation, and electron microscopy experiments, we established that Goliath is present in mitochondria of the R2C Leydig cell line. Using short-term hypophysectomized animals, we showed that Goliath is regulated by LH/hCG in Leydig cells but not in germ cells. This regulation in Leydig cells concerned only the 50-kDa isoform. This report is the first description of a differential regulation of the Goliath protein between germ cells and Leydig cells.

1 Supported by a grant from the Ministère de la Recherche et de la Technologie to A.G.

2 Correspondence: F. Bulle, Unité INSERM 581, Hôpital Henri Mondor, 94010 Créteil, France. FAX: 33 1 48 98 09 08; bulle{at}im3.inserm.fr






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Copyright © 2004 by the Society for the Study of Reproduction.