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BOR - Papers in Press, published online ahead of print October 15, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.021972
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BIOLOGY OF REPRODUCTION 70, 400–405 (2004)
DOI: 10.1095/biolreprod.103.021972
© 2004 by the Society for the Study of Reproduction, Inc.

Embryo

Developmental Potential of Bovine Androgenetic and Parthenogenetic Embryos: A Comparative Study1

Irina Lagutina, Giovanna Lazzari, Roberto Duchi, and Cesare Galli2

Laboratorio di Tecnologie della Riproduzione, Istituto Sperimentale Italiano Lazzaro Spallanzani, CIZ srl 26100 Cremona, Italy

In this study, we compared the developmental capacity of bovine haploid and diploid androgenetic and parthenogenetic embryos obtained by different methods. Androgenetic embryos were produced by piezo-intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) of enucleated oocytes with or without subsequent pronuclear transfer from one haploid zygote to another. Parthenogenetic embryos were obtained by activation of matured oocytes by ionomycin combined with cycloheximide or 6-dimethylaminopurine (DMAP) treatment. Only few cleaved androgenetic haploid embryos were able to compact (2.7%) and to form blastocysts (1.8%), while significantly more haploid parthenogenotes underwent compaction (24–37%) and a minority developed to blastocysts at different rates, depending on the activation procedure (cycloheximide 3%, 6-DMAP 14.5%). By contrast, development to blastocyst of diploid androgenotes, cloned androgenetic embryos, and parthenogenotes (31%, 39%, and 43%, respectively) was similar to IVF control embryos (35%). Cell number on Day 7 was higher for IVF blastocysts and decreased in consecutive order in diploid androgenotes, diploid parthenogenotes, and haploid uniparental embryos. Following transfer of diploid androgenetic embryos, a pregnancy was established and maintained up to Day 28.

1 Supported by MIPAF-RAIZ, FIRB-MIUR.

2 Correspondence: Cesare Galli, Laboratorio di Tecnologie della Riproduzione, CIZ srl, Via Porcellasco 7/f, 26100 Cremona, Italy. FAX: 39 0372 436133; cesare{at}galli2.191.it




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[Abstract] [Full Text] [PDF]


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