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BOR - Papers in Press, published online ahead of print October 1, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.020271
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BIOLOGY OF REPRODUCTION 70, 415–418 (2004)
DOI: 10.1095/biolreprod.103.020271
© 2004 by the Society for the Study of Reproduction, Inc.

Reproductive Technology

Nuclear Transfer of Adult Bone Marrow Mesenchymal Stem Cells: Developmental Totipotency of Tissue-Specific Stem Cells from an Adult Mammal1

Yoko Kato3, Hideaki Imabayashi4, Taisuke Mori4, Tetsuya Tani3, Masanori Taniguchi5, Mikihiko Higashi5, Michio Matsumoto5, Akihiro Umezawa4, and Yukio Tsunoda2,3

Laboratory of Animal Reproduction,3 College of Agriculture, Kinki University, Nara 631-8505, Japan Department of Pathology,4 Keio University School of Medicine, Tokyo 160-8582, Japan Kumamoto Prefecture Livestock Stations and Animal Public Health Center,5 Kumamoto 861-1113, Japan

Recent studies have demonstrated that somatic stem cells have a flexible potential greater than previously expected when they are transplanted into different tissues. On the other hand, recent studies also have revealed that these potentials might occur because of spontaneous cell fusion with recipient cells. The nuclei of somatic cells could have been reprogrammed when they were artificially or spontaneously fused with mouse embryonic stem (ES) cells. The resultant hybrid cells acquired a developmental pluripotency that the original somatic cells did not have but that ES cells did. LaBarge and Blau (Cell 2002; 111:589–601) demonstrated that adult bone marrow-derived cells contributed to muscle tissue in a stepwise biological progression. This means that bone marrow-derived cells became satellite cells of mononucleate muscle stem cells after the first irradiation-induced damage to the mouse, and after the second irradiation-induced damage, multinucleate myofibers appeared from the bone marrow-derived cells. Considered together, the differentiation potential of the somatic stem cell nucleus itself remains unclear. Although the pluripotency of somatic stem cell populations has been evaluated, the developmental totipotency of the nuclei of somatic stem cells, whether or not they fused with other cells, has not been shown, except in only one study concerning fetal neural cells (never in adult stem cells). Here, we showed the developmental totipotency of adult bovine mesenchymal stem cells by nuclear transfer.

1 Supported by grants from the Programme for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and partly supported by a grant from the Ministry of Education, Science, and Culture (14034259, 12358014, 13308050, 15039233); the Ministry of Agriculture, Forestry and Fisheries (Cloning); Ito Kinen Foundation; and Nakajima International Interchange Foundation.

2 Correspondence. FAX: 81 7 4243 115; tsunoda{at}nara.kindai.ac.jp




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