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Expression of Trophoblast Cell-Specific Pregnancy-Related Genes in SomaticCell-Cloned Bovine Pregnancies¹

National Institute of Agrobiological Sciences,⁴ Tsukuba, Ibaraki 305-8602 Japan National Institute of Livestock and Grassland Sciences,⁵ Tsukuba, Ibaraki 305-0901 Japan School of Veterinary Medicine,⁶ University of Wisconsin, Madison, Wisconsin 53706

We compared the expression of bovine prolactin-related protein-1 (bPRP-1), placental lactogen (bPL), and pregnancy-associated glycoproteins-1 (bPAG-1) and -9 (bPAG-9) genes in artificially inseminated (AI) and nuclear transferred (NT) cows during the first trimester of gestation using real-time reverse transcription-polymerase chain reaction and in situ hybridization. Placentomal (cotyledonary, caruncular) and interplacentomal (intercotyledonary, intercaruncular) tissues of AI and NT cows carrying either motile (M) or immotile (IM) fetuses were examined. Transcripts for bPL and bPAG-9 were lower (P < 0.01) in the fetal membranes of NT (n = 4) cows at Day 30 of gestation, compared with AI (n = 4) cows. There was no difference in the mean (± SEM) levels of expressions of bPRP-1, bPL, and PAG-1 in the placentomal and interplacentomal tissues of AI (n = 5) and NT (M, n = 4) cows at Day 60 of gestation. The mRNAs for bPRP-1, bPL, bPAG-1, and bPAG-9 genes were higher (P < 0.01) in the caruncular tissue of AI cows, compared with NT (IM, n = 4) cows at Day 60 of gestation. Expression of bPRP-1, bPL, bPAG-1, and bPAG-9 in the placentomal and interplacentomal tissues of the NT (n = 3) group varied considerably more, compared with the AI (n = 4) group at Day 100 of gestation. These findings suggest defective binucleate cell-specific gene transcriptional commands in NT cows.

¹ This research was supported by the Organized Research Combination System (ORCS) from the Education, Science, and Technology Agency and the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. O.V.P. was supported by an STA fellowship from JST.

² Correspondence: Kazuyoshi Hashizume, Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan. FAX: 81 19 621 6212; kazuha{at}iwate-u.ac.jp

³ Current address: School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan

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