| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Pregnancy |
State Key Laboratory of Reproductive Biology,3 Institute of Zoology, Chinese Academy of Sciences,Beijing 100080, China
Department of Obstetrics and Gynecology,4 Second College of China Medical University, Shenyang 110004, China
Embryonic implantation is a temporally and spatially restricted process that involves a precise cross talk between the embryo and the receptive maternal endometrium. Underlying the complex changes in the uterus during implantation is the alteration in gene expression pattern, which is not fully understood for the primates. In the present study, suppression subtractive hybridization (SSH) was performed to screen genes that were differentially expressed in the implantation site of the pregnant rhesus monkey, and a subtractive cDNA library was constructed. Furthermore, with dot blot analysis, reverse Northern blot analysis, and semiquantitative reverse transcription-polymerase chain reaction, 76 of 376 clones randomly selected from the library were proven to be differentially expressed in the implantation site. With DNA sequencing and BLAST analysis against the GenBank/EMBL database, it was demonstrated that the cDNA fragments carried by 73 clones shared high homology with 31 human genes. Among them, 15 positive clones represented the S100A10 gene and 10 positive ones corresponded with the secreted frizzled-related protein 4 gene. The other two clones shared homology with one human EST. There was one clone homologous to a human DNA sequence, which indicated that it might be a novel gene. To our knowledge, this is the first report to determine genes involved in the early implantation stage in the rhesus monkey with high throughput technology.
2 Correspondence: Yan-Ling Wang, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Haidian, Beijing 100080, China. FAX: 86 10 62529248; wangyl{at}panda.ioz.ac.cn
This article has been cited by other articles:
|
|
 |
|
  J. Liu, D. Li, B. Cao, Y.-x. Li, R. Herva, Y.-s. Piao, and Y.-l. Wang Expression and Localization of SWAP-70 in Human Fetomaternal Interface and Placenta During Tubal Pregnancy and Normal Placentation J. Histochem. Cytochem., July 1, 2007; 55(7): 701 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Momozawa, Y. Takeuchi, M. Kitago, K. Masuda, Y. Kakuma, C. Hashizume, T. Ichimaru, K. Mogi, H. Okamura, T. Yonezawa, et al. Gene Expression Profiles Linked to the Hormonal Induction of Male-Effect Pheromone Synthesis in Goats (Capra hircus) Biol Reprod, July 1, 2007; 77(1): 102 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. El-Sayed, M. Hoelker, F. Rings, D. Salilew, D. Jennen, E. Tholen, M.-A. Sirard, K. Schellander, and D. Tesfaye Large-scale transcriptional analysis of bovine embryo biopsies in relation to pregnancy success after transfer to recipients Physiol Genomics, December 13, 2006; 28(1): 84 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bauersachs, S. E Ulbrich, K. Gross, S. E M Schmidt, H. H D Meyer, H. Wenigerkind, M. Vermehren, F. Sinowatz, H. Blum, and E. Wolf Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity. Reproduction, August 1, 2006; 132(2): 319 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Klein, S. Bauersachs, S. E. Ulbrich, R. Einspanier, H. H.D. Meyer, S. E.M. Schmidt, H.-D. Reichenbach, M. Vermehren, F. Sinowatz, H. Blum, et al. Monozygotic Twin Model Reveals Novel Embryo-Induced Transcriptome Changes of Bovine Endometrium in the Preattachment Period Biol Reprod, February 1, 2006; 74(2): 253 - 264. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
