HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 70/5/1411    most recent biolreprod.103.023788v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zheng, P. Articles by Latham, K. E. Search for Related Content PubMed PubMed Citation Articles by Zheng, P. Articles by Latham, K. E. Agricola Articles by Zheng, P. Articles by Latham, K. E.

The Primate Embryo Gene Expression Resource: A Novel Resource to Facilitate Rapid Analysis of Gene Expression Patterns in Non-Human Primate Oocytes and Preimplantation Stage Embryos¹

The Fels Institute for Cancer Research and Molecular Biology³ the Department of Biochemistry,⁴Temple University School of Medicine, Philadelphia, Pennsylvania 19140 The Wisconsin National Primate Research Center,⁵ University of Wisconsin-Madison, Madison, Wisconsin 53715

Detailed molecular studies of preimplantation stage development in a suitable nonhuman primate model organism have been inhibited due to the cost and scarcity of embryos. To circumvent these limitations, we have created a new resource for the research community, designated as the Primate Embryo Gene Expression Resource (PREGER). The PREGER sample collection currently contains over 160 informative samples of oocytes, obtained from various sized antral follicles, and embryos obtained through a variety of different protocols. The PREGER makes it possible to undertake quantitative gene-expression studies in rhesus monkey oocytes and embryos through simple and cost-effective hybridization-based methods. The PREGER also makes available other molecular tools to facilitate nonhuman primate embryology. We used PREGER here to compare the temporal expression patterns of five housekeeping mRNAs and three transcription factor mRNAs between mouse and rhesus monkey. We observed noticeable differences in temporal expression patterns between species for some mRNAs, but clear similarities for others. Our results also provide new information related to genome activation and the effects of embryo culture conditions on gene expression in primate embryos. These results provide one illustration of how the PREGER can be employed to obtain novel insight into primate embryogenesis.

¹ Supported by a grant from the NIH/NCRR (RR15253) to K.E.L.

² Correspondence: Keith E. Latham, 3307 North Broad Street, Philadelphia, PA 19140. FAX: 215 707 1454. klatham{at}temple.edu

This article has been cited by other articles:

				L. Hao, R. Vassena, G. Wu, Z. Han, Y. Cheng, K. E. Latham, and C. Sapienza The Unfolded Protein Response Contributes to Preimplantation Mouse Embryo Death in the DDK Syndrome Biol Reprod, May 1, 2009; 80(5): 944 - 953. [Abstract] [Full Text] [PDF]

				J. K. Nyholt de Prada, Y. S. Lee, K. E. Latham, C. L. Chaffin, and C. A. VandeVoort Role for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro Am J Physiol Endocrinol Metab, May 1, 2009; 296(5): E1049 - E1058. [Abstract] [Full Text] [PDF]

				Y. S. Lee, K. E. Latham, and C. A. VandeVoort Effects of in vitro maturation on gene expression in rhesus monkey oocytes Physiol Genomics, October 8, 2008; 35(2): 145 - 158. [Abstract] [Full Text] [PDF]

				N. R Mtango and K. E Latham Differential Expression of Cell Cycle Genes in Rhesus Monkey Oocytes and Embryos of Different Developmental Potentials Biol Reprod, February 1, 2008; 78(2): 254 - 266. [Abstract] [Full Text] [PDF]

				N. R. Mtango and K. E. Latham Ubiquitin proteasome pathway gene expression varies in rhesus monkey oocytes and embryos of different developmental potential Physiol Genomics, September 11, 2007; 31(1): 1 - 14. [Abstract] [Full Text] [PDF]

				P. Zheng, R. Vassena, and K. E. Latham Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos Mol. Hum. Reprod., June 1, 2007; 13(6): 361 - 371. [Abstract] [Full Text] [PDF]

				P. Zheng, R. D. Schramm, and K. E. Latham Developmental Regulation and In Vitro Culture Effects on Expression of DNA Repair and Cell Cycle Checkpoint Control Genes in Rhesus Monkey Oocytes and Embryos Biol Reprod, June 1, 2005; 72(6): 1359 - 1369. [Abstract] [Full Text] [PDF]

				P. Zheng, B. Patel, M. McMenamin, E. Moran, A. M. Paprocki, M. Kihara, R. D. Schramm, and K. E. Latham Effects of Follicle Size and Oocyte Maturation Conditions on Maternal Messenger RNA Regulation and Gene Expression in Rhesus Monkey Oocytes and Embryos Biol Reprod, April 1, 2005; 72(4): 890 - 897. [Abstract] [Full Text] [PDF]

				M. F. Pera and A. O. Trounson Human embryonic stem cells: prospects for development Development, November 15, 2004; 131(22): 5515 - 5525. [Abstract] [Full Text] [PDF]

				P. Zheng, B. Patel, M. McMenamin, A. M. Paprocki, R. D. Schramm, N. G. Nagl Jr, D. Wilsker, X. Wang, E. Moran, and K. E. Latham Expression of Genes Encoding Chromatin Regulatory Factors in Developing Rhesus Monkey Oocytes and Preimplantation Stage Embryos: Possible Roles in Genome Activation Biol Reprod, May 1, 2004; 70(5): 1419 - 1427. [Abstract] [Full Text] [PDF]