Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print January 28, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.025510
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
70/6/1552    most recent
biolreprod.103.025510v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shi, F.
Right arrow Articles by LaPolt, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shi, F.
Right arrow Articles by LaPolt, P. S.
Agricola
Right arrow Articles by Shi, F.
Right arrow Articles by LaPolt, P. S.
BIOLOGY OF REPRODUCTION 70, 1552–1561 (2004)
DOI: 10.1095/biolreprod.103.025510
© 2004 by the Society for the Study of Reproduction, Inc.

Ovary

Cell-Specific Expression and Regulation of Soluble Guanylyl Cyclase {alpha}1 and ß1 Subunits in the Rat Ovary1

Fangxiong Shi, Robert L. Stewart, Jr, Emerson Perez, Jean Y.-H. Chen, and Philip S. LaPolt2

Department of Biological Sciences, California State University–Los Angeles, Los Angeles, California 90032

Soluble guanylyl cyclase (sGC) is activated by nitric oxide (NO) and carbon monoxide, resulting in cGMP production. Recent studies indicate that NO and cGMP influence ovarian functions. However, little information is available regarding the ovarian expression of sGC. The present study examined sGC {alpha}1 and ß1 subunit protein levels in the ovary during postnatal development, gonadotropin-induced follicle growth, ovulation, and luteinization as well as in cultured rat granulosa cells. In postnatal rats, sGC {alpha}1 subunit immunoreactivity was high in granulosa cells of primordial and primary follicles on Day 5 but low in granulosa cells of larger follicles on Days 10 and 19. Theca cells of developing follicles, but not stromal cells, also demonstrated moderate sGC {alpha}1 immunoreactivity. In gonadotropin- treated immature rats, intense sGC {alpha}1 subunit staining was similarly observed in granulosa cells of primordial and primary follicles, but such staining was low in granulosa cells of small antral follicles and undetectable in granulosa cells of large antral and preovulatory follicles. Following ovulation, corpora lutea expressed moderate sGC {alpha}1 immunoreactivity. Similar ovarian localization and expression patterns were seen for sGC ß1, indicating regulated coexpression of sGC subunits. Immunoblot analysis revealed no change in total ovarian sGC {alpha}1 and ß1 subunit protein levels during gonadotropin treatment. Similarly, no effect of FSH on sGC subunit protein levels was apparent in cultured granulosa cells. These findings indicate regulated, cell- specific patterns of sGC expression in the ovary and are consistent with roles for cGMP in modulating ovarian functions.

1 Supported by NIH SCORE Grant S06 GM008101 to P.S.L.

2 Correspondence: Philip S. LaPolt, Department of Biological Sciences, California State University–Los Angeles, 5151 State University Drive, Los Angeles, CA 90032. FAX: 323 343 6451;plapolt{at}exchange.calstatela.edu




This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
R. Huang, F. Shi, T. Lei, Y. Song, C. L. Hughes, and G. Liu
Effect of the Isoflavone Genistein Against Galactose-Induced Cataracts in Rats
Experimental Biology and Medicine, January 1, 2007; 232(1): 118 - 125.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
G. S. Palanisamy, Y.-P. Cheon, J. Kim, A. Kannan, Q. Li, M. Sato, S. R. Mantena, R. L. Sitruk-Ware, M. K. Bagchi, and I. C. Bagchi
A Novel Pathway Involving Progesterone Receptor, Endothelin-2, and Endothelin Receptor B Controls Ovulation in Mice
Mol. Endocrinol., November 1, 2006; 20(11): 2784 - 2795.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A. T Grazul-Bilska, C. Navanukraw, M. L. Johnson, D. A Arnold, L. P Reynolds, and D. A Redmer
Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle.
Reproduction, October 1, 2006; 132(4): 579 - 587.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. A Vonnahme, D. A Redmer, E. Borowczyk, J. J Bilski, J. S Luther, M. L. Johnson, L. P Reynolds, and A. T Grazul-Bilska
Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2{alpha}-induced regression in sheep.
Reproduction, June 1, 2006; 131(6): 1115 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
V. Sriraman, M. D. Rudd, S. M. Lohmann, S. M. Mulders, and J. S. Richards
Cyclic Guanosine 5'-Monophosphate-Dependent Protein Kinase II Is Induced by Luteinizing Hormone and Progesterone Receptor-Dependent Mechanisms in Granulosa Cells and Cumulus Oocyte Complexes of Ovulating Follicles
Mol. Endocrinol., February 1, 2006; 20(2): 348 - 361.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
F. Shi and T. Wang
Stage- and Cell-Specific Expression of Soluble Guanylyl Cyclase Alpha and Beta Subunits, cGMP-Dependent Protein Kinase I Alpha and Beta, and Cyclic Nucleotide-Gated Channel Subunit 1 in the Rat Testis
J Androl, March 1, 2005; 26(2): 258 - 263.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for the Study of Reproduction.