HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 70/6/1664    most recent biolreprod.103.023317v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Demeestere, I. Articles by Delbaere, A. Search for Related Content PubMed PubMed Citation Articles by Demeestere, I. Articles by Delbaere, A. Agricola Articles by Demeestere, I. Articles by Delbaere, A.

Effect of Insulin-Like Growth Factor-I During Preantral Follicular Culture on Steroidogenesis, In Vitro Oocyte Maturation, and Embryo Development in Mice¹

Research Laboratory on Human Reproduction and Fertility Clinic³ Laboratory of Chemistry,⁴ Erasme Hospital, French Speaking Free University of Brussels, 1070 Brussels, Belgium

Insulin-like growth factor-I (IGF-I) is involved in the regulation of ovarian follicular development and has been shown to potentiate the FSH responsiveness of granulosa cells from preantral follicles. The aim of the present study was to investigate the effect of IGF-I during preantral follicular culture on steroidogenesis, subsequent oocyte maturation, fertilization, and embryo development in mice. Preantral follicles were isolated mechanically and cultured for 12 days in a simplified culture medium supplemented with 1% fetal calf serum, recombinant human FSH, transferrin, and selenium. In these conditions, follicles were able to grow and produce oocytes that could be matured and fertilized. The first experiment analyzed the effect of different concentrations of IGF-I (0, 10, 50, or 100 ng/ml) added to the culture medium on the follicular survival, steroidogenesis, and the oocyte maturation process. The presence of IGF-I during follicular growth increased the secretion of estradiol but had no effect on the subsequent oocyte survival and maturation rates. In the second experiment, IGF-I (0 or 50 ng/ml) was added to the culture medium during follicular growth, oocyte maturation, or both, and subsequent oocyte fertilization and embryo development rates were evaluated. Oocyte fertilization rates were comparable in the presence or absence of IGF-I. However, the blastocyst development rate was enhanced after follicular culture in the presence of IGF-I. Moreover, the total cell number of the blastocysts observed after differential labeling staining was also higher when follicles were cultured or matured in the presence of IGF-I.

¹ This study was supported by the Belgian National Fund for Scientific Research.

² Correspondence: I. Demeestere, Research Laboratory on Human Reproduction, Erasme Hospital, 808 Route de Lennik, 1070 Brussels, Belgium. FAX: 0032 2 5554520; idemeest{at}ulb.ac.be

This article has been cited by other articles:

				O. Lacham-Kaplan, H. Chy, and A. Trounson Testicular Cell Conditioned Medium Supports Differentiation of Embryonic Stem Cells into Ovarian Structures Containing Oocytes Stem Cells, February 1, 2006; 24(2): 266 - 273. [Abstract] [Full Text] [PDF]

				I Demeestere, J Centner, C Gervy, Y Englert, and A Delbaere Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents Reproduction, August 1, 2005; 130(2): 147 - 156. [Abstract] [Full Text] [PDF]

				A. S. Chang, A. N. Dale, and K. H. Moley Maternal Diabetes Adversely Affects Preovulatory Oocyte Maturation, Development, and Granulosa Cell Apoptosis Endocrinology, May 1, 2005; 146(5): 2445 - 2453. [Abstract] [Full Text] [PDF]