Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print February 11, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.027177
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
70/6/1679    most recent
biolreprod.103.027177v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zilli, L.
Right arrow Articles by Vilella, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zilli, L.
Right arrow Articles by Vilella, S.
Agricola
Right arrow Articles by Zilli, L.
Right arrow Articles by Vilella, S.
BIOLOGY OF REPRODUCTION 70, 1679–1684 (2004)
DOI: 10.1095/biolreprod.103.027177
© 2004 by the Society for the Study of Reproduction, Inc.

Male Reproductive Tract

Adenosine Triphosphate Concentration and ß-D-Glucuronidase Activity as Indicators of Sea Bass Semen Quality

Loredana Zilli2, Roberta Schiavone2, Vincenzo Zonno3, Carlo Storelli2, and Sebastiano Vilella1,2

Laboratorio di Fisiologia Generale e Comparata Dipartimento di Scienze e Tecnologie Biologiche e Ambientali,2 Università di Lecce, 73100 Lecce, Italy Marine Aquaculture and Fisheries Research Centre,3 Frigole-Lecce, 73100 Lecce, Italy

The most common parameters used to evaluate sperm quality are motility rate and duration and fertilization ability. In this study, chemical and biochemical parameters of sea bass (Dicentrarchus labrax) sperm were investigated to find an alternative method for evaluating sperm fertilization ability before and after cryopreservation. The biochemical and chemical analyses were performed with fresh and frozen-thawed sperm and seminal plasma. To cryopreserve sperm, 250-µl straws were used. Fertilization ability was evaluated by inseminating eggs (obtained from hormonally stimulated females) with fresh and cryopreserved sperm. The results revealed a linear relationship (P < 0.05) between semen fertilization capacity and some seminal plasma (ß-D-glucuronidase activity, potassium concentration) and sperm (ATP concentration, aspartate aminotransferase activity) parameters. Variations in semen fertilization rate could be best described by two multiple regression models: one including the sperm parameters and another including the seminal plasma parameters. For practical application, the use of simple regression models is of value. Fertilization rate in both fresh and cryopreserved sperm was reliably predicted by determining the ATP concentration or the ß-D-glucuronidase activity or both.

1 Correspondence: Sebastiano Vilella, Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Laboratorio di Fisiologia Generale e Comparata, Università di Lecce, Via provinciale per Monteroni, 73100 Lecce, Italy. FAX: 39 0832 324220; sebastiano.vilella{at}unile.it




This article has been cited by other articles:


Home page
 ReproductionHome page
J. Cosson, A.-L. Groison, M. Suquet, C. Fauvel, C. Dreanno, and R. Billard
Marine fish spermatozoa: racing ephemeral swimmers
Reproduction, September 1, 2008; 136(3): 277 - 294.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. Zilli, R. Schiavone, V. Zonno, R. Rossano, C. Storelli, and S. Vilella
Effect of Cryopreservation on Sea Bass Sperm Proteins
Biol Reprod, May 1, 2005; 72(5): 1262 - 1267.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for the Study of Reproduction.