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BOR - Papers in Press, published online ahead of print February 11, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.026666
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BIOLOGY OF REPRODUCTION 70, 1782–1789 (2004)
DOI: 10.1095/biolreprod.103.026666
© 2004 by the Society for the Study of Reproduction, Inc.

Testis

Testicular Activity of Mos in the Frog, Rana esculenta: A New Role in Spermatogonial Proliferation1

Diana Ferrara3, Carmela Palmiero3, Margherita Branno4, Riccardo Pierantoni2,3, and Sergio Minucci3

Dipartimento di Medicina Sperimentale-sezione "F. Bottazzi,"3 Seconda Università degli Studi di Napoli, 80138 Napoli, Italy Laboratori di Biochimica e Biologia Molecolare,4 Stazione Zoologica "A. Dohrn"-Villa Comunale 121, 80132 Napoli, Italy

Mos is a MAPK kinase kinase with an expression that is highly restricted to the gonads. Its function is mainly associated to the meiotic metaphase II arrest occurring during female gametogenesis, whereas to our knowledge, its role during spermatogenesis has not yet clarified. In the present paper, we report the isolation of c-mos cDNA and the identification of a 60-kDa Mos protein from the testis of the anuran amphibian, Rana esculenta. Both the transcript and the protein are always present at low levels in the testis during the frog annual sexual cycle, with single significant peaks of expression in March and May, respectively. Mos is mainly localized in the cytoplasm of primary and secondary spermatogonia (SPG). Therefore, we have used treatments with ethane-dimethane sulphonate (EDS), which blocks spermatogonial mitosis in frogs. Four days after a single EDS injection, Mos expression in SPG highly increases concomitantly with the temporary arrest of mitosis. From 8 to 28 days after the injection, the normal proliferative activity of SPG is restored, and Mos expression gradually decreases to control levels. These results strongly indicate that the c-mos proto-oncogene exerts a new role associated to the regulation of spermatogonial proliferation.

1 Supported by grants from MURST "ex 40% PRIN" and "Ricerca di Ateneo ex 60%."

2 Correspondence. FAX: 39 081 5667536; riccardo.pierantoni{at}unina2.it






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Copyright © 2004 by the Society for the Study of Reproduction.