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This Article Full Text Full Text (PDF) All Versions of this Article: 71/1/194    most recent biolreprod.104.027706v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Haigo, K. Articles by Horiuchi, T. Search for Related Content PubMed PubMed Citation Articles by Haigo, K. Articles by Horiuchi, T. Agricola Articles by Haigo, K. Articles by Horiuchi, T.

Full-Term Development of Hamster Embryos Produced by Injection of Round Spermatids into Oocytes

Graduate School of Applied Biosciences,² Hiroshima Prefectural University, Hiroshima, 727-0023 Japan The Institute for Biogenesis Research,³ University of Hawaii School of Medicine, Honolulu, Hawaii 96822

The golden hamster is a mammal in which microinjection of round spermatids into oocytes (ROSI) was first attempted. However, no live ROSI offspring have ever been obtained in this species. This is the first report of live hamster offspring obtained by round spermatid injection. Over 90% of oocytes, injected with round spermatids, were activated without any additional stimulation. The proportion of the oocytes that were fertilized normally and that developed to morulae and blastocysts was higher when the plasma membranes of the spermatids were broken before injection, as compared with when the membranes were left intact. Five percent of 57 ROSI morulae/blastocysts developed into live offspring after transfer to foster mothers.

¹ Correspondence: Toshitaka Horiuchi, Department of Bioresources, Hiroshima Prefectural University, Shoubara, Hiroshima, 727-0023 Japan. FAX: 81 8247 4 1750; toshi{at}bio.hiroshima-pu.ac.jp

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