HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 71/1/282    most recent biolreprod.103.024125v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thompson, W. E. Articles by Tsang, B. K. Search for Related Content PubMed PubMed Citation Articles by Thompson, W. E. Articles by Tsang, B. K. Agricola Articles by Thompson, W. E. Articles by Tsang, B. K.

Regulation of Prohibitin Expression During Follicular Development and Atresia in the Mammalian Ovary¹

Department of Obstetrics & Gynecology and Cooperative Reproductive Science Research Center,³ Department of Microbiology Biochemistry and Immunology,⁴ Morehouse School of Medicine, Atlanta, Georgia 30310 Department of Obstetrics & Gynecology and Cellular & Molecular Medicine,⁵ University of Ottawa and Ottawa Health Research Institute, Ottawa, Canada K1Y 4E9 Departments of Animal Sciences⁶ Obstetrics and Gynecology,⁷ University of Missouri-Columbia, Columbia, Missouri 65211-5300 Departement de Chimie-Biologie,⁸ Section de Biologie-Medicale, Universite du Quebec a Trois-Rivieres, Trois-Rivieres, Quebec, Canada G9A 5H7

Prohibitin is a ubiquitous and highly conserved protein implicated as an important regulator in cell survival. Prohibitin content is inversely associated with cell proliferation, but it increases during granulosa cell differentiation as well as in earlier events of apoptosis in a temperature-sensitive granulosa cell line. In the present study, we have characterized the spatial expression patterns for prohibitin using established in vivo models for the induction of follicular development and atresia in the mammalian ovary. Comparative Western blot analyses of granulosa cell lysates from control ovaries and from ovaries primed with eCG or treated with eCG plus anti-eCG (gonadotropin withdrawal) were conducted. Prohibitin was immunolocalized in rat ovarian sections probed with antibodies against either proliferating cell nuclear antigen (PCNA) or cholesterol side-chain cleavage cytochrome P450 (P450_scc) or in terminal deoxynucleotidyl transferase-mediated dUTP nick end labeled sections. Additionally, porcine oocytes, zygotes, and blastocyts were also immunolocalized with prohibitin antibody. Immunolocalization revealed the presence of prohibitin in granulosa cells, theca-interstitial cells, and the oocyte. The results indicate that prohibitin protein expression in the gonadotropin-treated cells was upregulated. Immunoreactivity of prohibitin was inversely related to PCNA expression during follicular maturation and colocalized with P450_scc. Prohibitin appeared to be translocated from the cytoplasm to the nucleus in atretic follicles, germinal vesicle-stage oocytes, zygotes, and blastocysts. These results suggest that prohibitin has several functional regulatory roles in granulosa and theca-interstitial cells and in the ovum during follicular maturation and atresia. It is likely that prohibitin may play an important role in determining the fate of these cells and eventual follicular destiny.

¹ Supported, in part, by grants from the National Institutes of Health (GM08248, RR03034, HD41749, and NCI P50-CA83591 SPORE in Ovarian Cancer to W.E.T.; RR13438 to R.S.P.), USDA (99-35203-11743 and 2002-02069 to P.S.), the F21C program of the University of Missouri-Columbia (P.S., R.S.P., and E.R.), and the Canadian Institutes of Health Research (MOP-10369 to B.K.T.). This work was presented, in part, at the 35th annual meeting of the Society for the Study of Reproduction in Baltimore, Maryland, July 27–31, 2002.

² Correspondence: Winston E. Thompson, Department of Obstetrics & Gynecology, Cooperative Reproductive Science Research Center, Morehouse School of Medicine, 720 Westview Drive Southwest, Atlanta, GA 30310. FAX: 404 752 1754; thompsw{at}msm.edu

This article has been cited by other articles:

				I. Chowdhury, W. Xu, J. K. Stiles, A. Zeleznik, X. Yao, R. Matthews, K. Thomas, and W. E. Thompson Apoptosis of Rat Granulosa Cells after Staurosporine and Serum Withdrawal Is Suppressed by Adenovirus-Directed Overexpression of Prohibitin Endocrinology, January 1, 2007; 148(1): 206 - 217. [Abstract] [Full Text] [PDF]

				X. Peng, R. Mehta, S. Wang, S. Chellappan, and R. G. Mehta Prohibitin is a novel target gene of vitamin d involved in its antiproliferative action in breast cancer cells. Cancer Res., July 15, 2006; 66(14): 7361 - 7369. [Abstract] [Full Text] [PDF]

				S. Rastogi, B. Joshi, G. Fusaro, and S. Chellappan Camptothecin Induces Nuclear Export of Prohibitin Preferentially in Transformed Cells through a CRM-1-dependent Mechanism J. Biol. Chem., February 3, 2006; 281(5): 2951 - 2959. [Abstract] [Full Text] [PDF]

				M. R. Hussein Apoptosis in the ovary: molecular mechanisms Hum. Reprod. Update, March 1, 2005; 11(2): 162 - 178. [Abstract] [Full Text] [PDF]