HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 71/1/73    most recent biolreprod.103.025205v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bobe, J. Articles by Jalabert, B. Search for Related Content PubMed PubMed Citation Articles by Bobe, J. Articles by Jalabert, B. Agricola Articles by Bobe, J. Articles by Jalabert, B.

Targeted Gene Expression Profiling in the Rainbow Trout (Oncorhynchus mykiss) Ovary During Maturational Competence Acquisition and Oocyte Maturation

Institut National de la Recherche Agronomique, SCRIBE, Campus de Beaulieu, 35042 Rennes Cedex, France

A real-time polymerase chain reaction-based gene expression survey was performed using 37 target genes and 22 female rainbow trout sampled during follicular maturational competence (FMC) acquisition or during oocyte maturation. In females sampled before meiosis resumption, FMC was estimated using an in vitro assay. Several growth factors, bone morphogenetic proteins, steroidogenic enzymes, cathepsins, genes known to play a role in the fish preovulatory ovary, as well as previously unstudied genes, were analyzed in this survey. Gene expression profiling was performed using a supervised clustering analysis in order to identify groups of genes exhibiting similar expression profiles in the ovary during FMC acquisition and follicular maturation. From the clustering analysis, three clusters exhibiting a specific expression during FMC acquisition or at the time of oocyte maturation were identified. Cluster 1 was characterized by a progressive increase in gene expression during FMC acquisition, whereas cluster 2 exhibited an increased expression at the time of oocyte maturation. In contrast, cluster 3 was characterized by a decreased mRNA expression at the time of oocyte maturation. Among the 37 target genes used in this survey, 18 were significantly regulated during maturational competence acquisition or at the time of oocyte maturation. Among these 18 genes, 16 belonged to one of the three clusters identified. Although the results allowed a global description of gene expression profiles, they also suggest an important role for several factors, including some previously unstudied bone morphogenetic proteins, in the paracrine control of FMC acquisition and meiosis resumption.

¹ Correspondence: Julien Bobe, Institut National de la Recherche Agronomique, SCRIBE, Campus de Beaulieu, 35042 Rennes Cedex, France. FAX: 33 2 23 48 50 20; bobe{at}beaulieu.rennes.inra.fr

This article has been cited by other articles:

				V Tripathi and A Krishna Changes in nitric oxide (NO) synthase isoforms and NO in the ovary of Heteropneustes fossilis (Bloch.) during the reproductive cycle J. Endocrinol., November 1, 2008; 199(2): 307 - 316. [Abstract] [Full Text] [PDF]

				J. Bobe, S. Mahe, T. Nguyen, H. Rime, D. Vizziano, A. Fostier, and Y. Guiguen A Novel, Functional, and Highly Divergent Sex Hormone-Binding Globulin that May Participate in the Local Control of Ovarian Functions in Salmonids Endocrinology, June 1, 2008; 149(6): 2980 - 2989. [Abstract] [Full Text] [PDF]

				B. Campbell, J. Dickey, B. Beckman, G. Young, A. Pierce, H. Fukada, and P. Swanson Previtellogenic Oocyte Growth in Salmon: Relationships among Body Growth, Plasma Insulin-Like Growth Factor-1, Estradiol-17beta, Follicle-Stimulating Hormone and Expression of Ovarian Genes for Insulin-Like Growth Factors, Steroidogenic-Acute Regulatory Protein and Receptors for Gonadotropins, Growth Hormone, and Somatolactin Biol Reprod, July 1, 2006; 75(1): 34 - 44. [Abstract] [Full Text] [PDF]

				M Kusakabe, I Nakamura, J Evans, P Swanson, and G Young Changes in mRNAs encoding steroidogenic acute regulatory protein, steroidogenic enzymes and receptors for gonadotropins during spermatogenesis in rainbow trout testes. J. Endocrinol., June 1, 2006; 189(3): 541 - 554. [Abstract] [Full Text] [PDF]

				B. B. Kamangar, J.-C. Gabillard, and J. Bobe Insulin-Like Growth Factor-Binding Protein (IGFBP)-1, -2, -3, -4, -5, and -6 and IGFBP-Related Protein 1 during Rainbow Trout Postvitellogenesis and Oocyte Maturation: Molecular Characterization, Expression Profiles, and Hormonal Regulation Endocrinology, May 1, 2006; 147(5): 2399 - 2410. [Abstract] [Full Text] [PDF]

				S. Milla, B. Jalabert, H. Rime, P. Prunet, and J. Bobe Hydration of rainbow trout oocyte during meiotic maturation and in vitro regulation by 17,20{beta}-dihydroxy-4-pregnen-3-one and cortisol J. Exp. Biol., March 15, 2006; 209(6): 1147 - 1156. [Abstract] [Full Text] [PDF]

				G. M. Weber and C. V. Sullivan Insulin-Like Growth Factor-I Induces Oocyte Maturational Competence but Not Meiotic Resumption in White Bass (Morone chrysops) Follicles In Vitro: Evidence for Rapid Evolution of Insulin-Like Growth Factor Action Biol Reprod, May 1, 2005; 72(5): 1177 - 1186. [Abstract] [Full Text] [PDF]