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BOR - Papers in Press, published online ahead of print April 7, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.027342
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BIOLOGY OF REPRODUCTION 71, 464–469 (2004)
DOI: 10.1095/biolreprod.104.027342
© 2004 by the Society for the Study of Reproduction, Inc.

Neuroendocrinology

Evidence for Different Gonadotropin-Releasing Hormone Response Sites in Rat Ovarian and Pituitary Cells1

Lucas A. Mongiat3, Victoria A. Lux-Lantos3, and Carlos Libertun2,3,4

Laboratorio de Neuroendocrinología,3 Instituto de Biología y Medicina Experimental (IBYME), 1428 Buenos Aires, Argentina Departamento de Fisiología,4 Facultad de Medicina, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina

The participation of type I GnRH receptor (GnRH-R) on GnRH-II-induced gonadotropin secretion in rat pituitary cells was investigated. Furthermore, we extended the study of GnRH-II action to ovarian cells. The GnRH-II was able to mobilize inositol triphosphate (IP3) and to induce LH and FSH release in a dose-dependent manner in pituitary cells and in a GnRH-I-like manner. The GnRH-analog 135-18 (agonist for type II GnRH-R and antagonist for type I GnRH-R) was unable to elicit any cellular response tested in these pituitary cells. The GnRH-II responses were blocked by the type I GnRH-R-antagonists CRX or 135-18, suggesting that these effects were mediated by the type I GnRH-R. In contrast to pituitary cells, GnRH-I, but not GnRH-II, elicited an IP3 response in superovulated ovarian cells; 135-18 also had no effect. However, GnRH-II as well as GnRH-I presented antiproliferative effects on these cells. Surprisingly, 135-18 had stronger antiproliferative effects than either GnRH peptide. The 135-18 analog, but not GnRH-I or GnRH-II, increased progesterone secretion in superovulated ovarian cells. These results strongly suggest that GnRH-II is able to stimulate rat pituitary cells through the type I GnRH-R, with no evidence for the presence of type II GnRH-R. On the other hand, our results indicate a putative GnRH-R in superovulated ovarian cells with response characteristics that differ from those of the GnRH-R in the pituitary.

1 Supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT, BID 1201/OC AR PICT 2000, 05 08664 to C.L.), Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 02231 to V.A.L.-L. and PIP 02282 to C.L.), Universidad de Buenos Aires (M045 to C.L.), and Ministerio de Salud de la Nación (to V.A.L.-L.).

2 Correspondence: Carlos Libertun, Instituto de Biología y Medicina Experimental—Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina. FAX: 54 011 4786 2564; libertun{at}dna.uba.ar






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Copyright © 2004 by the Society for the Study of Reproduction.