HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 71/2/515    most recent biolreprod.104.027565v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kwon, J. Articles by Yoshikawa, Y. Search for Related Content PubMed PubMed Citation Articles by Kwon, J. Articles by Yoshikawa, Y. Agricola Articles by Kwon, J. Articles by Yoshikawa, Y.

Developmental Regulation of Ubiquitin C-Terminal Hydrolase Isozyme Expression During Spermatogenesis in Mice

Department of Biomedical Science,² Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan Department of Degenerative Neurological Disease,³ National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, 187-8502, Japan Laboratory of Pathophysiology,⁴ Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, 812-8582, Japan

The ubiquitin pathway functions in the process of protein turnover in eukaryotic cells. This pathway comprises the enzymes that ubiquitinate/deubiquitinate target proteins and the proteasome that degrades ubiquitin-conjugated proteins. Ubiquitin C-terminal hydrolases (UCHs) are thought to be essential for maintaining ubiquitination activity by releasing ubiquitin (Ub) from its substrates. Mammalian UCH-L1 and UCH-L3 are small proteins that share considerable homology at the amino acid level. Both of these UCHs are highly expressed in the testis/ ovary and neuronal cells. Our previous work demonstrated that UCH-L1-deficient gracile axonal dystrophy (gad) mice exhibit progressively decreasing spermatogonial stem cell proliferation, suggesting that UCH isozymes in the testis function during spermatogenesis. To analyze the expression patterns of UCH isozymes during spermatogenesis, we isolated nearly homogeneous populations of spermatogonia, spermatocytes, spermatids, and Sertoli cells from mouse testes. Western blot analysis detected UCH-L1 in spermatogonia and Sertoli cells, whereas UCH-L3 was detected in spermatocytes and spermatids. Moreover, reverse transcription-polymerase chain reaction analysis of UCH isozymes showed that UCH-L1 and UCH-L4 mRNAs are expressed in spermatogonia, whereas UCH-L3 and UCH-L5 mRNAs are expressed mainly in spermatocytes and spermatids. These results suggest that UCH-L1 and UCH-L3 have distinct functions during spermatogenesis, namely, that UCH-L1 may act during mitotic proliferation of spermatogonial stem cells whereas UCH-L3 may function in the meiotic differentiation of spermatocytes into spermatids.

¹ Correspondence: Yasuhiro Yoshikawa, Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. FAX: 81 3 5841 8186; ayyoshi{at}mail.ecc.u-tokyo.ac.jp

This article has been cited by other articles:

				X. Yu, S. Hong, and E. M. Faustman Cadmium-induced Activation of Stress Signaling Pathways, Disruption of Ubiquitin-dependent Protein Degradation and Apoptosis in Primary Rat Sertoli Cell-Gonocyte Cocultures Toxicol. Sci., August 1, 2008; 104(2): 385 - 396. [Abstract] [Full Text] [PDF]

				M. B. Butterworth, R. S. Edinger, H. Ovaa, D. Burg, J. P. Johnson, and R. A. Frizzell The Deubiquitinating Enzyme UCH-L3 Regulates the Apical Membrane Recycling of the Epithelial Sodium Channel J. Biol. Chem., December 28, 2007; 282(52): 37885 - 37893. [Abstract] [Full Text] [PDF]

				Y.-J. Yi, G. Manandhar, M. Sutovsky, R. Li, V. Jonakova, R. Oko, C.-S. Park, R. S Prather, and P. Sutovsky Ubiquitin C-Terminal Hydrolase-Activity Is Involved in Sperm Acrosomal Function and Anti-Polyspermy Defense During Porcine Fertilization Biol Reprod, November 1, 2007; 77(5): 780 - 793. [Abstract] [Full Text] [PDF]

				B. Seliger, A. Fedorushchenko, W. Brenner, A. Ackermann, D. Atkins, S. Hanash, and R. Lichtenfels Ubiquitin COOH-Terminal Hydrolase 1: A Biomarker of Renal Cell Carcinoma Associated with Enhanced Tumor Cell Proliferation and Migration[?Q1: Running head: UCHL1, a Biomarker of RCC. Short title OK?Q1] Clin. Cancer Res., January 1, 2007; 13(1): 27 - 37. [Abstract] [Full Text] [PDF]

				S. Sekiguchi, J. Kwon, E. Yoshida, H. Hamasaki, S. Ichinose, M. Hideshima, M. Kuraoka, A. Takahashi, Y. Ishii, S. Kyuwa, et al. Localization of Ubiquitin C-Terminal Hydrolase L1 in Mouse Ova and Its Function in the Plasma Membrane to Block Polyspermy Am. J. Pathol., November 1, 2006; 169(5): 1722 - 1729. [Abstract] [Full Text] [PDF]

				Y. Sano, A. Furuta, R. Setsuie, H. Kikuchi, Y.-L. Wang, M. Sakurai, J. Kwon, M. Noda, and K. Wada Photoreceptor Cell Apoptosis in the Retinal Degeneration of Uchl3-Deficient Mice Am. J. Pathol., July 1, 2006; 169(1): 132 - 141. [Abstract] [Full Text] [PDF]

				J. Kwon, K. Mochida, Y.-L. Wang, S. Sekiguchi, T. Sankai, S. Aoki, A. Ogura, Y. Yoshikawa, and K. Wada Ubiquitin C-Terminal Hydrolase L-1 Is Essential for the Early Apoptotic Wave of Germinal Cells and for Sperm Quality Control During Spermatogenesis Biol Reprod, July 1, 2005; 73(1): 29 - 35. [Abstract] [Full Text] [PDF]

				J. Kwon, Y.-L. Wang, R. Setsuie, S. Sekiguchi, Y. Sato, M. Sakurai, M. Noda, S. Aoki, Y. Yoshikawa, and K. Wada Two Closely Related Ubiquitin C-Terminal Hydrolase Isozymes Function as Reciprocal Modulators of Germ Cell Apoptosis in Cryptorchid Testis Am. J. Pathol., October 1, 2004; 165(4): 1367 - 1374. [Abstract] [Full Text] [PDF]