Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print April 28, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.027235
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
71/3/714    most recent
biolreprod.104.027235v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guzeloglu Kayisli, O.
Right arrow Articles by Arici, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guzeloglu Kayisli, O.
Right arrow Articles by Arici, A.
Agricola
Right arrow Articles by Guzeloglu Kayisli, O.
Right arrow Articles by Arici, A.
BIOLOGY OF REPRODUCTION 71, 714–721 (2004)
DOI: 10.1095/biolreprod.104.027235
© 2004 by the Society for the Study of Reproduction, Inc.

Female Reproductive Tract

In Vivo and In Vitro Regulation of Akt Activation in Human Endometrial Cells Is Estrogen Dependent

Ozlem Guzeloglu Kayisli2,3, Umit A. Kayisli2,4, Guven Luleci3, and Aydin Arici1,2

Division of Reproductive Endocrinology,2 Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06520-8063 Departments of Medical Biology and Genetics3 Histology and Embryology,4 School of Medicine, Akdeniz University, Antalya 07070, Turkey

Estrogen-bound estrogen receptors (ER) {alpha} and ß classically activate gene expression after binding to the estrogen response element in the promoter regions of target genes. Estrogen also has rapid, nongenomic effects. It activates several membranous or cytoplasmic kinase cascades, including the phosphatidylinositol 3-phosphate (PI3K/Akt) cascade, a signaling pathway that plays a key role in cell survival and apoptosis. Normal human endometrium is exposed to variable levels of steroid hormones throughout the menstrual cycle. We hypothesized that Akt phosphorylation in human endometrium may vary with the menstrual cycle and in early pregnancy and that fluctuations in estrogen level may play a role in Akt activation in endometrial cells. We analyzed Akt phosphorylation using in vivo and in vitro techniques, including Western blot, immunohistochemistry, and immunocytochemistry. Estradiol significantly increased Akt phosphorylation in endometrial cells. Rapid stimulation of Akt activation in cultured stromal cells was observed. Akt phosphorylation by estradiol was inhibited by the PI3K inhibitor, wortmannin, but not by the ER antagonist, ICI 182 780. The maximal effect on Akt activity was observed following 5–15 min of estradiol treatment. Our results suggest that estradiol may directly affect PI3K-related signaling pathway by increasing the phosphorylation of Akt in endometrial cells. Thus, estradiol may exert part of its proliferative and antiapoptotic effects by a nongenomic manner through the Akt signaling pathway.

1 Correspondence: FAX: 203 785 7134; aydin.arici{at}yale.edu






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for the Study of Reproduction.