Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print May 12, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.028159
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BIOLOGY OF REPRODUCTION 71, 926–932 (2004)
DOI: 10.1095/biolreprod.104.028159
© 2004 by the Society for the Study of Reproduction, Inc.

Testis

Expression and Functional Significance of Mouse Paraspeckle Protein 1 on Spermatogenesis1

Reiko Myojin3, Sho Kuwahara4, Tomohiko Yasaki4, Takahito Matsunaga3, Takayuki Sakurai5, Minoru Kimura5, Seiichi Uesugi4, and Yasuyuki Kurihara2,4

Department of Chemistry and Bioengineering,3 Graduate School of Engineering, Department of Environment and Natural Sciences,4 Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama 240-0851, Japan Department of Genetic Information,5 School of Medicine, Tokai University, Isehara 259-1153, Japan

Paraspeckle protein 1 (PSP1) in humans is a recently identified component protein of a novel nuclear body, paraspeckle. The protein has a DBHS (Drosophila behavior, human splicing) motif that is found in PSF and p54nrb/NonO proteins. These DBHS-containing proteins have been reported to be involved in various nuclear events such as DNA replication, transcription, and mRNA processing. Here we show that mouse paraspeckle protein 1 (mPSP1; encoded by the Pspc1 gene) has two isoforms with different C-termini lengths. Abundant expression of the longer isoform (mPSP1-{alpha}) and the shorter one (mPSP1-ß) were observed in testis and kidney, respectively. Transiently expressed mPSP1-{alpha} was localized in nuclei, but mPSP1-ß was localized in both nuclei and cytoplasm. These observations suggest that alternative splicing regulates tissue distribution and subcellular localization. Like other DBHS-containing proteins, mPSP1 has RNA-binding activity. In mouse testis, mPSP1-{alpha} was found in the nuclear matrix fraction. Furthermore, by coimmunoprecipitation, we confirmed that mPSP1 interacts with other DBHS-containing proteins, PSF and p54nrb/NonO. Therefore, we conclude that mPSP1 may regulate multiple phases of important nuclear events during spermatogenesis.

1 This work was supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan; the Kanagawa Academy of Science and Technology Research; and the Kihara Memorial Yokohama Foundation. R.M. and S.K. contributed equally to this work.

2 Correspondence: Yasuyuki Kurihara, Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, Tokiwa-dai, Hodogaya, Yokohama 240-8501, Japan. FAX: 81 45 339 4263; kurihara{at}mac.bio.bsk.ynu.ac.jp






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Copyright © 2004 by the Society for the Study of Reproduction.