Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print May 19, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.024422
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
71/3/973    most recent
biolreprod.103.024422v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishizono, H.
Right arrow Articles by Nakagata, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishizono, H.
Right arrow Articles by Nakagata, N.
Agricola
Right arrow Articles by Nishizono, H.
Right arrow Articles by Nakagata, N.
BIOLOGY OF REPRODUCTION 71, 973–978 (2004)
DOI: 10.1095/biolreprod.103.024422
© 2004 by the Society for the Study of Reproduction, Inc.

Male Reproductive Tract

Decrease of Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury1

Hirofumi Nishizono3,4, Masaki Shioda5, Toru Takeo6, Tetsumi Irie6, and Naomi Nakagata2,4

Kyudo Company Limited,3 Kumamoto 861-0104, Japan Center for Animal Resources and Development,4 Kumamoto University, Kumamoto 860-0811, Japan Department of Materials and Life Science,5 Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan Department of Clinical Chemistry and Informatics,6 Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8555, Japan

In general, the fertilizing ability of cryopreserved mouse spermatozoa is less than that of fresh spermatozoa. This ability is especially low in C57BL/6, the main strain used for the production of transgenic mice. To solve this problem, the relationship between cell damage and fertilizing ability in cryopreserved mouse spermatozoa was examined in this study. Sperm motility analysis revealed no significant difference among the motilities of cryopreserved C57BL/6J, BALB/cA, and DBA/2N sperm (67.6%, 43.4%, and 60.0%, respectively) after thawing. However, the results of in vitro fertilization (IVF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) showed a strong correlation between the frequency of aberrant spermatozoa (FAS) and fertilization rates (FR; C57BL/6J: FAS, 83.7%; FR, 17.0%; BALB/cA: FAS, 67.2%; FR, 24.2%; and DBA/2N: FAS, 10.2%; FR, 93.6%), and damage to spermatozoa was localized particularly in the acrosome of the head and mitochondria.

1 Supported by a Grant-in-Aid for Scientific Research, 11177101-04, from the Japan Society for the Promotion of Science.

2 Correspondence: Naomi Nakagata, 2-2-1 Honjo, Kumamoto 860-0811, Japan. FAX: +81 96 373 6560; nakagata{at}gpo.kumamoto-u.ac.jp






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for the Study of Reproduction.