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Spam1 (PH-20) Expression in the Extratesticular Duct and Accessory Organs of the Mouse: A Possible Role in Sperm Fluid Reabsorption¹

Department of Biological Sciences,³ University of Delaware, Newark, Delaware 19711 Department of Anatomy and Cell Biology,⁴ McGill University, Montreal, Quebec, Canada H3A 2B2 Centre Hospitalier de l'Universite Laval,⁵ Ste Foy, Quebec, Canada G1V 4G2

A widely conserved sperm antigen, the sperm adhesion molecule 1 (SPAM1 or PH-20) is a glycosylphosphatidyl inositol-linked protein with multiple roles in mammalian fertilization. It has been shown to be dually expressed in testis and epididymis and this is conserved in the four species (mouse, rat, macaques, humans) that have been studied to date. Here, we report Spam1 RNA and protein expression in the murine vas deferens and efferent ducts. In situ hybridization and immunohistochemistry indicate that transcript and protein are distributed in the nonciliated epithelial cells and that the efferent ducts have the most intense staining of all three regions of the excurrent ducts. Spam1 products were also present in the accessory organs, the prostate, and seminal vesicles and its fluid. Using hyaluronic acid substrate gel electrophoresis, hyaluronidase activity at pH 7.0 was detected in the vas deferens but was absent from the efferent ducts, the prostate, and the seminal vesicles/fluid. This suggests that Spam1 may play a nonenzymatic role in these organs. In the efferent ducts, where Spam1 is enriched in the apical (but not basolateral) membrane of nonciliated cells, it is likely to play a role in sperm concentration, which is the established function of that organ.

¹ Supported by NIH grant RO1 HD38273 to P.A.M.-D.

² Correspondence. FAX: 302 831 2281; pdeleon{at}udel.edu

This article has been cited by other articles:

				K. A. Miller, M. Shao, and P. A. Martin-Deleon Hyalp1 in Murine Sperm Function: Evidence for Unique and Overlapping Functions With Other Reproductive Hyaluronidases J Androl, January 1, 2007; 28(1): 67 - 76. [Abstract] [Full Text] [PDF]