HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 71/4/1142    most recent biolreprod.103.024828v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thomas, R.E. Articles by Gilchrist, R.B. Search for Related Content PubMed PubMed Citation Articles by Thomas, R.E. Articles by Gilchrist, R.B. Agricola Articles by Thomas, R.E. Articles by Gilchrist, R.B.

Effect of Specific Phosphodiesterase Isoenzyme Inhibitors During In Vitro Maturation of Bovine Oocytes on Meiotic and Developmental Capacity¹

Research Centre for Reproductive Health, Department of Obstetrics and Gynaecology, University of Adelaide, The Queen Elizabeth Hospital, 5011, Adelaide, Australia

Compared with oocytes matured in vivo, in vitro-matured oocytes are compromised in their capacity to support early embryo development. Delaying spontaneous in vitro meiotic maturation using specific phosphodiesterase (PDE) isoenzyme inhibitors may permit more complete oocyte cytoplasmic maturation, possibly by prolonging cumulus cell (CC)-oocyte gap junctional communication during meiotic resumption. This study aimed to investigate the effect of the isoenzyme 3- (oocyte) and isoenzyme 4- (granulosa cell) specific PDE inhibitors on the kinetics of in vitro maturation and on subsequent oocyte developmental competence. Cumulus-oocyte complexes from antral bovine follicles were isolated and cultured in the presence of the specific PDE inhibitors milrinone (type 3) or rolipram (type 4) (100 µM). In the presence of FSH, both PDE inhibitors only slightly extended CC-oocyte gap junctional communication over the first 9 h, but they completely blocked meiotic resumption during this period (P < 0.001). The indefinite inhibitory effect of milrinone on meiotic resumption (30% at germinal vesicle stage after 48 h) was overridden by 24 h when treated with FSH, but not with hCG, suggesting a form of induced meiotic resumption. Oocytes treated with FSH with or without either PDE inhibitor were inseminated at either 24, 26, or 28 h. Treated with either the type 3 or type 4 PDE inhibitor significantly (P < 0.05) increased embryo development to the blastocyst stage by 33%–39% (to an average of 52% blastocysts) compared with control oocytes (38%) after insemination at 28 h, and significantly (P < 0.05) increased blastocyst cell numbers when inseminated at 24 h. These results suggest that delayed spontaneous meiotic maturation, coupled with extended gap junctional communication between the CCs and the oocyte has a positive effect on oocyte cytoplasmic maturation, thereby improving oocyte developmental potential.

¹ Supported by an Australian Postgraduate Award Scholarship to R.E.T., and in part by the FTT Fricker Medical Research Associateship from the University of Adelaide to R.B.G.

² Correspondence. FAX: 61 8 8222 7521; robert.gilchrist{at}adelaide.edu.au

This article has been cited by other articles:

				M. Sasseville, M.-C. Gagnon, C. Guillemette, R. Sullivan, R. B. Gilchrist, and F. J. Richard Regulation of Gap Junctions in Porcine Cumulus-Oocyte Complexes: Contributions of Granulosa Cell Contact, Gonadotropins, and Lipid Rafts Mol. Endocrinol., May 1, 2009; 23(5): 700 - 710. [Abstract] [Full Text] [PDF]

				L. Vanhoutte, D. Nogueira, F. Dumortier, and P. De Sutter Assessment of a new in vitro maturation system for mouse and human cumulus-enclosed oocytes: three-dimensional prematuration culture in the presence of a phosphodiesterase 3-inhibitor Hum. Reprod., April 23, 2009; (2009) dep104v1. [Abstract] [Full Text] [PDF]

				L. Vanhoutte, D. Nogueira, and P. De Sutter Prematuration of human denuded oocytes in a three-dimensional co-culture system: effects on meiosis progression and developmental competence Hum. Reprod., March 1, 2009; 24(3): 658 - 669. [Abstract] [Full Text] [PDF]

				R. B. Gilchrist, M. Lane, and J. G. Thompson Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality Hum. Reprod. Update, March 1, 2008; 14(2): 159 - 177. [Abstract] [Full Text] [PDF]

				Y.-m. Shu, H.-t. Zeng, Z. Ren, G.-l. Zhuang, X.-y. Liang, H.-w. Shen, S.-z. Yao, P.-q. Ke, and N.-n. Wang Effects of cilostamide and forskolin on the meiotic resumption and embryonic development of immature human oocytes Hum. Reprod., March 1, 2008; 23(3): 504 - 513. [Abstract] [Full Text] [PDF]

				D. Nogueira, R. Ron-El, S. Friedler, M. Schachter, A. Raziel, R. Cortvrindt, and J. Smitz Meiotic Arrest In Vitro by Phosphodiesterase 3-Inhibitor Enhances Maturation Capacity of Human Oocytes and Allows Subsequent Embryonic Development Biol Reprod, January 1, 2006; 74(1): 177 - 184. [Abstract] [Full Text] [PDF]

				D Nogueira, R Cortvrindt, B Everaerdt, and J Smitz Effects of long-term in vitro exposure to phosphodiesterase type-3 inhibitors on follicle and oocyte development Reproduction, August 1, 2005; 130(2): 177 - 186. [Abstract] [Full Text] [PDF]