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This Article Full Text Full Text (PDF) All Versions of this Article: 71/4/1202    most recent biolreprod.104.031294v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kanatsu-Shinohara, M. Articles by Shinohara, T. Search for Related Content PubMed PubMed Citation Articles by Kanatsu-Shinohara, M. Articles by Shinohara, T. Agricola Articles by Kanatsu-Shinohara, M. Articles by Shinohara, T.

Transgenic Mice Produced by Retroviral Transduction of Male Germ Line Stem Cells In Vivo¹

Horizontal Medical Research Organization³ Department of Pathology and Biology of Diseases,⁴ Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan

Spermatogonial stem cells are the only stem cells in the postnatal body that can transmit parental genetic information to the offspring, making them an attractive target cell population for animal transgenesis. Although transgenic mice and rats were recently produced by retrovirus transduction of these cells in vitro, with transplantation of the transduced cells into infertile recipients, the difficulty of restoring fertility and preparing recipients using spermatogonial transplantation limits practical application of the technique. In this article, we describe a novel approach for producing transgenic animals by transducing spermatogonial stem cells in vivo using a retrovirus vector. Microinjection of retrovirus into immature seminiferous tubules resulted in the direct transduction of spermatogonial stem cells in situ, and the animals produced transgenic offspring after mating with females. Transgenic mice were produced in C57BL/6, BALB/C, A, and C3H backgrounds, with an average efficiency of 2.8%. The transgene was transmitted stably and expressed in the next generation. The technique overcomes the drawback of the in vitro-transduction approach, and will be useful as a novel method for producing transgenic animals as well as providing a means for analyzing the self-renewal and differentiation processes of spermatogonial stem cells in vivo.

¹ Support for this research was provided by the Ministry of Education, Culture, Sports, Science and Technology of Japan.

² Correspondence: Takashi Shinohara, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan. FAX: 81 75 753 9306; takashi{at}mfour.med.kyoto-u.ac.jp

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