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This Article Full Text Full Text (PDF) All Versions of this Article: 71/4/1296    most recent biolreprod.104.028621v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zaga, V. Articles by Vadillo-Ortega, F. Search for Related Content PubMed PubMed Citation Articles by Zaga, V. Articles by Vadillo-Ortega, F. Agricola Articles by Zaga, V. Articles by Vadillo-Ortega, F.

Secretions of Interleukin-1ß and Tumor Necrosis Factor by Whole Fetal Membranes Depend on Initial Interactions of Amnion or Choriodecidua with Lipopolysaccharides or Group B Streptococci¹

Biomedical Research Branch,³ Direction of Research,⁴ Obstetrics Gynecology Branch,⁵ and Neonatology Branch,⁶ Instituto Nacional de Perinatologia, Mexico City 11000, Mexico Experimental Medicine Department,⁷ School of Medicine, Universidad Nacional Autonoma de Mexico, Mexico City 04510, Mexico

The present study evaluated the secretions of interleukin (IL)-1ß and tumor necrosis factor (TNF) by fetal membranes stimulated with group B streptococci (GBS) and lipopolysaccharide (LPS). The aim was to evaluate the initial response of full-thickness membranes to the microbial insult using an in vitro experimental model that allowed testing of the individual contributions of amnion and choriodecidua to stimulation. Full-thickness membranes were obtained after delivery by elective cesarean section from women at 37–40 wk of gestation without evidence of active labor. The membranes were mounted in Transwell devices, physically separating the upper and lower chambers. The LPS (500 ng/ml) or GBS (1 x 10⁶ colony-forming units/ml) was added to either the amniotic or choriodecidual surface, and accumulation of IL-1ß and TNF were measured in both compartments using a specific ELISA. Fetal membranes followed different patterns of secretion of proinflammatory cytokines that depended on the side to which the stimulus was added or the nature of the stimulus itself. The TNF was secreted by amnion and choriodecidua in the presence of LPS or GBS, and stimulation with GBS induced a greater synthesis of IL-1ß than did stimulation with LPS. Choriodecidual tissue was more responsive than amniotic tissue, and this response tended to be higher even when the stimulation was only on the amniotic side. However, the amnion plays an active role in recognizing LPS or GBS, contributing a significant amount of TNF. Thus, cooperative and bidirectional communications occur between amnion and choriodecidua in response to bacterial products, which include intermembranous cytokine traffic and signaling between tissues.

¹ Supported by a grant from the Consejo Nacional de Ciencia y Tecnología (CONACyT- 21117), Mexico.

² Correspondence: Felipe Vadillo-Ortega, Instituto Nacional de Perinatologia, Montes Urales 800, Lomas de Virreyes, Mexico D.F. 11000, Mexico. FAX: 52 5 5520 0034; felipe.vadillo{at}uia.mx

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