HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 71/5/1430    most recent biolreprod.104.031260v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kwon, I.-K. Articles by Niwa, K. Search for Related Content PubMed PubMed Citation Articles by Kwon, I.-K. Articles by Niwa, K. Agricola Articles by Kwon, I.-K. Articles by Niwa, K.

Activation, Pronuclear Formation, and Development In Vitro of Pig Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa¹

The Graduate School of Natural Science and Technology,³ Okayama University, Okayama 700-8530, Japan Department of Animal Science and Technology,⁴ Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan

The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa was evaluated. Activation and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried spermatozoa, but cleaved embryos were generally difficult to develop to the morula or blastocyst stage. When spermatozoa were freeze-dried for 24 h, oocyte activation and MPN formation in activated oocytes after sperm head injection were inhibited. Embryo development to the blastocyst stage was only obtained after injecting sperm heads isolated from spermatozoa freeze-dried for 4 h and stored at 4°C. The proportion of embryos that developed to the blastocyst stage was not increased by the treatment of injected oocytes with Ca ionophore (5–10 µM). Increasing the sperm storage time did not affect oocyte activation or MPN formation, but blastocyst development was observed only after 1 mo of storage. These results demonstrate that pig oocytes can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst stage.

¹ Supported in part by a Grant-in-Aid for Creative Scientific Research from the Japan Society for the Promotion of Science (13GS0008).

² Correspondence. FAX: 81 86 251 8388; kniwa{at}cc.okayama-u.ac.jp

This article has been cited by other articles:

				C. Li, E. Mizutani, T. Ono, and T. Wakayama Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI Reproduction, May 1, 2009; 137(5): 779 - 792. [Abstract] [Full Text] [PDF]

				E. Garcia-Rosello, C. Matas, S. Canovas, P. N. Moreira, J. Gadea, and P. Coy Influence of Sperm Pretreatment on the Efficiency of Intracytoplasmic Sperm Injection in Pigs J Androl, March 1, 2006; 27(2): 268 - 275. [Abstract] [Full Text] [PDF]

				K.-B. Lee and K. Niwa Fertilization and Development In Vitro of Bovine Oocytes Following Intracytoplasmic Injection of Heat-Dried Sperm Heads Biol Reprod, January 1, 2006; 74(1): 146 - 152. [Abstract] [Full Text] [PDF]

				M. Katayama, P. Sutovsky, B. S Yang, T. Cantley, A. Rieke, R. Farwell, R. Oko, and B. N Day Increased disruption of sperm plasma membrane at sperm immobilization promotes dissociation of perinuclear theca from sperm chromatin after intracytoplasmic sperm injection in pigs Reproduction, December 1, 2005; 130(6): 907 - 916. [Abstract] [Full Text] [PDF]

				L. K. McGinnis, L. Zhu, J. A. Lawitts, S. Bhowmick, M. Toner, and J. D. Biggers Mouse Sperm Desiccated and Stored in Trehalose Medium Without Freezing Biol Reprod, October 1, 2005; 73(4): 627 - 633. [Abstract] [Full Text] [PDF]