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BOR - Papers in Press, published online ahead of print June 30, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.030304
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BIOLOGY OF REPRODUCTION 71, 1533–1539 (2004)
DOI: 10.1095/biolreprod.104.030304
© 2004 by the Society for the Study of Reproduction, Inc.

Gamete Biology

Protein Patterns of Pig Oocytes During In Vitro Maturation1

Zdenka Ellederova3, Petr Halada4, Petr Man4, Michal Kubelka3, Jan Motlik3, and Hana Kovarova2,3

Institute of Animal Physiology and Genetics,3 Academy of Sciences of the Czech Republic, 277 21 Libechov, Czech Republic Institute of Microbiology,4 Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic

In vitro maturation (IVM) of fully grown mammalian oocytes is characterized by initial germinal vesicle (GV) breakdown and rearrangement of microtubule network during the first meiosis (MI), followed by extrusion of the first polar body and block of the oocytes in metaphase of the second meiosis (MII). Only fully matured oocytes are capable of undergoing fertilization and the initiation of zygotic development. These observations are mostly based on morphological evaluation; however, the molecular events responsible for these processes are not known. In this study, we have launched the analysis of pig oocytes during in vitro maturation using a proteomics approach. First, oocyte proteins have been separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Remarkably, several proteins, including peroxiredoxins, ubiquitin carboxyl-terminal hydrolase isozyme L1, and spermine synthase, are even more abundant than actin, usually the most abundant protein in somatic cells. Furthermore, we have initiated comparative analysis of the oocytes at different stages of maturation to characterize candidate proteins, which are differentially expressed during in vitro maturation. To date, we have identified antiquitin (D7A1), the member of aldehyde dehydrogenase family7 that has been significantly increased in MI and MII stages compared with GV oocytes. To our knowledge, this is the first pig oocyte proteome available so far that may be used as a reference map. The proteins that are differentially regulated during IVM may present potential biomarkers of oocyte maturation and quality. It is a useful inventory toward a deeper understanding of the mechanisms underlying reproduction and development.

1 Supported by CRSF research grant 204/04/0571, Ministry of Education grant LN 00A00650, and by Institutional Research Concept AV0Z5020903.

2 Correspondence: Hana Kovarova, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska str. 89, 277 21 Libechov, Czech Republic. FAX: 420 315 639 510; kovarova{at}iapg.cas.cz






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Copyright © 2004 by the Society for the Study of Reproduction.