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This Article Full Text Full Text (PDF) All Versions of this Article: 71/5/1591    most recent biolreprod.104.030585v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hayes, E.S. Articles by Unemori, E.N. Search for Related Content PubMed PubMed Citation Articles by Hayes, E.S. Articles by Unemori, E.N. Agricola Articles by Hayes, E.S. Articles by Unemori, E.N.

Implantation and Pregnancy Following In Vitro Fertilization and the Effect of Recombinant Human Relaxin Administration in Macaca fascicularis¹

Monash Institute of Reproduction and Development,⁵ Clayton, Victoria 3168, Australia Department of Pathology,⁶ University of New Mexico School of Medicine, Albuquerque, New Mexico 87131 BAS Medical, Inc.,⁷ San Mateo, California 94402

Implantation and early pregnancy, and the potential effects of the reproductive-hormone relaxin, were examined in the cynomolgus macaque (Macaca fascicularis) following in vitro fertilization and embryo transfer. Mature oocytes were collected from regularly cycling, female cynomolgus monkeys subjected to ovarian superovulation using recombinant human FSH and hCG. Oocytes fertilized in vitro were cultured to the 4- to 8-cell stage, slow-cooled, and stored in liquid nitrogen before thawing and embryo transfer. Regularly cycling recipients were administered recombinant human relaxin or vehicle for 21 days through the peri-implantation period (Day 0 = pump implantation), during which time the thawed embryos were transferred (Day 7). Endometrial thickness and the number of gestational sacs were monitored by ultrasound at three time points (Days 7, 21, and 28). The number of days of placental sign (implantation bleeding) in pregnant females and menses in nonpregnant females were also recorded. Implantation (gestational sacs/embryo transferred) and multiple pregnancy (multiple gestations/ pregnant recipient) rates were slightly higher in relaxin-treated recipients compared to vehicle-treated recipients. Administration of relaxin was associated with increased implantation bleeding in pregnant females. Endometrial thickness was increased in relaxin-treated recipients at Days 7 and 28 compared to Day 0, but these differences were not observed at the same time points in vehicle-treated females. Systemic administration of recombinant human relaxin in an in vitro fertilization/embryo transfer setting was associated with effects consistent with a role for this hormone in endometrial physiology in primates.

¹ Funded by Connetics Corp., Palo Alto, California.

² Correspondence: E.N. Unemori, BAS Medical, Inc., 1660 South Amphlett Blvd., Ste. 200, San Mateo, CA 94402. FAX: 650-235-3579; eunemori{at}basmedical.com

³ Current address: The Washington National Primate Research Center, University of Washington, Box 357331, Seattle, WA 98195

⁴ Current address: Monash Immunology and Stem Cell laboratories, Monash University, Clayton, VIC 3800, Australia