| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Ovary |
Prince Henry's Institute of Medical Research,3
Department of Biochemistry and Molecular Biology,4 Monash University, Clayton, Victoria 3168, Australia
MRC Human Reproductive Sciences Unit,5 Centre for Reproductive Biology, The Chancellors Building, University of Edinburgh, Edinburgh EH16 4SB, United Kingdom
Centre for Urological Research,6 Monash Institute of Reproduction and Development, Clayton, Victoria 3168, Australia
Estradiol-17ß (E2) affects late follicular development, whereas primordial follicle differentiation and early activation are believed to be independent of E2. To test this hypothesis we compared numbers of primordial and primary follicles in wild-type and E2-deficient, aromatase knockout (ArKO) mice, and the immunohistochemical staining or mRNA expression of Mullerian inhibiting substance (MIS), Wilms tumor 1 (WT-1), and growth differentiation factor (GDF9), which are known to effect early follicular differentiation. Proliferating cell nuclear antigen (PCNA) staining was a marker of proliferative index. The effects of E2 replacement for 3 wk in 7-wk-old ArKO and wild-type mice on these parameters were also tested. ArKO mice had reduced numbers of primordial and primary follicles compared with wild-type mice (63%, P < 0.001 and 60%, P = 0.062, respectively). This reduction was not corrected by E2 treatment, suggesting that E2 affects the initial formation or activation of primordial follicles. There was a significant increase in the diameters of the oocytes in primordial follicles of ArKO mice compared with mice of the wild type. There were no differences in the immunostaining of MIS, WT-1, and PCNA in primordial and primary follicles between wild-type and ArKO mice. The only difference was as a consequence of Sertoli and Leydig cells that develop in ovaries of ArKO mice. GDF9 mRNA expression was markedly increased in ArKO ovaries. E2 treatment restored the ovarian follicular morphology in ArKO mice, and consequently the immunostaining patterns, but had no effect on early follicle numbers. In conclusion, E2 has a role in controlling the size of the oocyte and primordial follicle pool in mice.
2 Correspondence: Kara Britt, Prince Henry's Institute of Medical Research, Monash Medical Centre Clayton, Block E, Level 4, Clayton, VIC 3168, Australia. FAX: 61 3 9594 6125; kara.britt{at}phimr.monash.edu.au
This article has been cited by other articles:
|
|
 |
|
  P. Smith, T. L. Steckler, A. Veiga-Lopez, and V. Padmanabhan Developmental Programming: Differential Effects of Prenatal Testosterone and Dihydrotestosterone on Follicular Recruitment, Depletion of Follicular Reserve, and Ovarian Morphology in Sheep Biol Reprod, April 1, 2009; 80(4): 726 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A McLaughlin and S. C McIver Awakening the oocyte: controlling primordial follicle development Reproduction, January 1, 2009; 137(1): 1 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wang, E. R. Prossnitz, and S. K. Roy G Protein-Coupled Receptor 30 Expression Is Required for Estrogen Stimulation of Primordial Follicle Formation in the Hamster Ovary Endocrinology, September 1, 2008; 149(9): 4452 - 4461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M Seneda, M. Godmann, B. D Murphy, S. Kimmins, and V. Bordignon Developmental regulation of histone H3 methylation at lysine 4 in the porcine ovary Reproduction, June 1, 2008; 135(6): 829 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Kipp, S. M. Kilen, T. K. Woodruff, and K. E. Mayo Activin Regulates Estrogen Receptor Gene Expression in the Mouse Ovary J. Biol. Chem., December 14, 2007; 282(50): 36755 - 36765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wang, E. R. Prossnitz, and S. K. Roy Expression of G Protein-Coupled Receptor 30 in the Hamster Ovary: Differential Regulation by Gonadotropins and Steroid Hormones Endocrinology, October 1, 2007; 148(10): 4853 - 4864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mayo, L. Jameson, and T. K. Woodruff Eggs in the Nest Endocrinology, August 1, 2007; 148(8): 3577 - 3579. [Full Text] [PDF]
|
|
|
|
|
|
J. L. Kipp, S. M. Kilen, S. Bristol-Gould, T. K. Woodruff, and K. E. Mayo Neonatal Exposure to Estrogens Suppresses Activin Expression and Signaling in the Mouse Ovary Endocrinology, May 1, 2007; 148(5): 1968 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wang and S. K. Roy Development of Primordial Follicles in the Hamster: Role of Estradiol-17{beta} Endocrinology, April 1, 2007; 148(4): 1707 - 1716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sahmi, E. S Nicola, and C. A Price Hormonal regulation of cytochrome P450 aromatase mRNA stability in non-luteinizing bovine granulosa cells in vitro. J. Endocrinol., July 1, 2006; 190(1): 107 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A Slot, J. Kastelijn, A. Bachelot, P. A Kelly, N. Binart, and K. J Teerds Reduced recruitment and survival of primordial and growing follicles in GH receptor-deficient mice. Reproduction, March 1, 2006; 131(3): 525 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L Juengel, D. A Heath, L. D Quirke, and K. P McNatty Oestrogen receptor {alpha} and {beta}, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep Reproduction, January 1, 2006; 131(1): 81 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Jefferson, R. Newbold, E. Padilla-Banks, and M. Pepling Neonatal Genistein Treatment Alters Ovarian Differentiation in the Mouse: Inhibition of Oocyte Nest Breakdown and Increased Oocyte Survival Biol Reprod, January 1, 2006; 74(1): 161 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Woodruff and K. E. Mayo To {beta} or Not To {beta}: Estrogen Receptors and Ovarian Function Endocrinology, August 1, 2005; 146(8): 3244 - 3246. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
