HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 71/6/1766    most recent biolreprod.104.029413v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wolf, D. P. Articles by Lester, L. Search for Related Content PubMed PubMed Citation Articles by Wolf, D. P. Articles by Lester, L. Agricola Articles by Wolf, D. P. Articles by Lester, L.

Progress with Nonhuman Primate Embryonic Stem Cells¹

Division of Reproductive Sciences,³ Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006 Department of Medicine,⁴ Oregon Health and Science University, Portland, Oregon 97201

Embryonic stem cells hold potential in the fields of regenerative medicine, developmental biology, tissue regeneration, disease pathogenicity, and drug discovery. Embryonic stem (ES) cell lines are now available in primates, including man, rhesus, and cynomologous monkeys. Monkey ES cells serve as invaluable clinically relevant models for studies that can't be conducted in humans because of practical or ethical limitations, or in rodents because of differences in physiology and anatomy. Here, we review the current status of nonhuman primate research with ES cells, beginning with a description of their isolation, characterization, and availability. Substantial limitations still plague the use of primate ES cells, such as their required growth on feeder layers, poor cloning efficiency, and restricted availability. The ability to produce homogenous populations of both undifferentiated as well as differentiated phenotypes is an important challenge, and genetic approaches to achieving these objectives are discussed. Finally, safety, efficiency, and feasibility issues relating to the transplantation of ES-derived cells are considered.

¹ Supported by National Institutes of Health grants RR15199, to D.P.W., HD18185 to Richard L. Stouffer (H-C.K), RR00163 to Peter Kohler (L.L.), and NS41601 to K.-Y.F.P.

² Correspondence: Don P. Wolf, Oregon National Primate Research Center, 505 NW 185th Ave., Beaverton, OR 97006. FAX: 503 533 2494; wolfd{at}ohsu.edu

This article has been cited by other articles:

				D. Rajesh, N. Chinnasamy, S. M. Mitalipov, D. P. Wolf, I. Slukvin, J. A. Thomson, and A. F. Shaaban Differential Requirements for Hematopoietic Commitment Between Human and Rhesus Embryonic Stem Cells Stem Cells, February 1, 2007; 25(2): 490 - 499. [Abstract] [Full Text] [PDF]

				K. Adachi, E. Kawase, K. Yasuchika, T. Sumi, N. Nakatsuji, and H. Suemori Establishment of the Gene-Inducible System in Primate Embryonic Stem Cell Lines Stem Cells, November 1, 2006; 24(11): 2566 - 2572. [Abstract] [Full Text] [PDF]

				A. L. Olsen, D. L. Stachura, and M. J. Weiss Designer blood: creating hematopoietic lineages from embryonic stem cells Blood, February 15, 2006; 107(4): 1265 - 1275. [Abstract] [Full Text] [PDF]

				A. Fujimoto, S.M. Mitalipov, L.L. Clepper, and D.P. Wolf Development of a monkey model for the study of primate genomic imprinting Mol. Hum. Reprod., June 1, 2005; 11(6): 413 - 422. [Abstract] [Full Text] [PDF]

				A. L. Glowinski Clone Being: Exploring the Psychological and Social Dimensions JAMA, September 22, 2004; 292(12): 1497 - 1498. [Full Text] [PDF]