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Gene Expression Profiling of Embryonic Stem Cells Leads to Greater Understanding of Pluripotency and Early Developmental Events¹

Rhodes Animal Science Center, University of Georgia, Athens, Georgia 30602-2771

Embryonic stem cells are characterized by their ability to propagate indefinitely in culture, maintaining a normal karyotype and their undifferentiated state. They have the potential of differentiating into any specialized cell type in the body. An understanding of the transcriptional profile related to pluripotency and early development is necessary to better tap their developmental potential and also maintain their undifferentiated phenotype. Currently, several techniques are in use to ascertain the gene expression profile of embryonic stem cells. This review summarizes the information generated using microarray and other approaches on the gene expression analyses of stem cells in both mouse and human cell lines. We also discuss specific approaches useful in future studies aimed at further deciphering the pluripotent nature of human embryonic stem cells.

¹ Funding has been provided by the Georgia Tech/Emory Center (GTEC) for the Engineering of Living Tissues, an ERC Program of the National Science Foundation under award EEC-9731643.

² Correspondence: Steven L. Stice, Rhodes Animal Science Center, University of Georgia, 425 River Road, Athens, GA 30602-2771. FAX: 706 542 7925; sstice{at}uga.edu

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