Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


BOR - Papers in Press, published online ahead of print August 25, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.030841
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
71/6/2079    most recent
biolreprod.104.030841v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ackerman, W. E.
Right arrow Articles by Kniss, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ackerman, W. E., IV
Right arrow Articles by Kniss, D. A.
Agricola
Right arrow Articles by Ackerman, W. E.
Right arrow Articles by Kniss, D. A.
BIOLOGY OF REPRODUCTION 71, 2079–2086 (2004)
DOI: 10.1095/biolreprod.104.030841
© 2004 by the Society for the Study of Reproduction, Inc.


Pregnancy

Epidermal Growth Factor and Interleukin-1ß Utilize Divergent Signaling Pathways to Synergistically Upregulate Cyclooxygenase-2 Gene Expression in Human Amnion-Derived WISH Cells1

William E. Ackerman, IV3, Brad H. Rovin4, and Douglas A. Kniss2,3

Department of Obstetrics and Gynecology (Division of Maternal-Fetal Medicine and Laboratory of Perinatal Research),3 Department of Internal Medicine (Division of Nephrology and Dorothy M. Davis Heart and Lung Research Institute),4 The Ohio State University, College of Medicine and Public Health, Columbus, Ohio 43210

In human parturition, uterotonic prostaglandins (PGs) arise predominantly via increased expression of cyclooxygenase-2 (COX-2 [also known as prostaglandin synthase 2]) within intrauterine tissues. Interleukin-1 (IL-1) and epidermal growth factor (EGF), both inducers of COX-2 transcription, are among numerous factors that accumulate within amniotic fluid with advancing gestation. It was previously demonstrated that EGF could potentiate IL-1ß-driven PGE2 production in amnion and amnion-derived (WISH) cells. To define the mechanism for this observation, we hypothesized that EGF and IL-1ß might exhibit synergism in regulating COX-2 gene expression. In WISH cells, combined treatment with EGF and IL-1ß resulted in a greater-than-additive increase in COX-2 mRNA relative to challenge with either agent independently. Augmentation of IL-1ß-induced transactivation by EGF was not observed in cells harboring reporter plasmids bearing nuclear factor-kappa B (NF{kappa}B) regulatory elements alone, but was evident when a fragment (–891/ +9) of the COX-2 gene 5'-promoter was present. Both agents transiently activated intermediates of multiple signaling pathways potentially involved in the regulation of COX-2 gene expression. The 26 S proteasome inhibitor, MG-132, selectively abrogated IL-1ß-driven NF{kappa}B activation and COX-2 mRNA expression. Only pharmacologic blockade of the p38 mitogen-activated protein kinase eliminated COX-2 expression following EGF stimulation. We conclude that EGF and IL-1ß appear to signal through different signaling cascades leading to COX-2 gene expression. IL-1ß employs the NF{kappa}B pathway predominantly, while the spectrum of EGF signaling is broader and includes p38 kinase. The synergism observed between IL-1ß and EGF does not rely on augmented NF{kappa}B function, but rather, occurs through differential use of independent response elements within the COX-2 promoter.

1 Supported in part by NIH grant RO1 HD35881 (D.A.K.) and The Ohio State University Perinatal Research and Development Fund. Portions of this work were presented at the 49th Annual Meeting of the Society for Gynecologic Investigation in Los Angeles, California, March 20–23, 2002, and the 51st Annual Meeting of the Society for Gynecologic Investigation in Houston, Texas, March 24–27, 2004.

2 Correspondence: Douglas A. Kniss, Laboratory of Perinatal Research, Department of Obstetrics and Gynecology, The Ohio State University, 5th Floor Means Hall, 1654 Upham Drive, Columbus, OH 43210. FAX: 614 293 5728; kniss.1{at}osu.edu




This article has been cited by other articles:


Home page
Biol. Reprod.Home page
W. E Ackerman IV, T. L.S Summerfield, D. D Vandre, J. M Robinson, and D. A Kniss
Nuclear Factor-Kappa B Regulates Inducible Prostaglandin E Synthase Expression in Human Amnion Mesenchymal Cells
Biol Reprod, January 1, 2008; 78(1): 68 - 76.
[Abstract] [Full Text] [PDF]


Home page
ReproductionHome page
G. Chevillard, A. Derjuga, D. Devost, H. H Zingg, and V. Blank
Identification of interleukin-1{beta} regulated genes in uterine smooth muscle cells
Reproduction, December 1, 2007; 134(6): 811 - 822.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
M. Liu, S.-C. Yang, S. Sharma, J. Luo, X. Cui, K. A. Peebles, M. Huang, M. Sato, R. D. Ramirez, J. W. Shay, et al.
EGFR Signaling Is Required for TGF-beta1 Mediated COX-2 Induction in Human Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 578 - 588.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
M. Mizuno, H. Sotoyama, E. Narita, H. Kawamura, H. Namba, Y. Zheng, T. Eda, and H. Nawa
A Cyclooxygenase-2 Inhibitor Ameliorates Behavioral Impairments Induced by Striatal Administration of Epidermal Growth Factor
J. Neurosci., September 19, 2007; 27(38): 10116 - 10127.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
M. M. Djavani, O. R. Crasta, J. C. Zapata, Z. Fei, O. Folkerts, B. Sobral, M. Swindells, J. Bryant, H. Davis, C. D. Pauza, et al.
Early Blood Profiles of Virus Infection in a Monkey Model for Lassa Fever
J. Virol., August 1, 2007; 81(15): 7960 - 7973.
[Abstract] [Full Text] [PDF]


Home page
J. Histochem. Cytochem.Home page
W. E. Ackerman IV, J. M. Robinson, and D. A. Kniss
Despite Transcriptional and Functional Coordination, Cyclooxygenase-2 and Microsomal Prostaglandin E Synthase-1 Largely Reside in Distinct Lipid Microdomains in WISH Epithelial Cells
J. Histochem. Cytochem., November 1, 2005; 53(11): 1391 - 1401.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
W. E. Ackerman IV, X. L. Zhang, B. H. Rovin, and D. A. Kniss
Modulation of Cytokine-Induced Cyclooxygenase 2 Expression by PPARG Ligands Through NF{kappa}B Signal Disruption in Human WISH and Amnion Cells
Biol Reprod, September 1, 2005; 73(3): 527 - 535.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for the Study of Reproduction.