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This Article Full Text Full Text (PDF) All Versions of this Article: 72/1/42    most recent biolreprod.104.033480v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, J. B. Articles by Yoon, H. S. Search for Related Content PubMed PubMed Citation Articles by Lee, J. B. Articles by Yoon, H. S. Agricola Articles by Lee, J. B. Articles by Yoon, H. S.

Establishment and Maintenance of Human Embryonic Stem Cell Lines on Human Feeder Cells Derived from Uterine Endometrium under Serum-Free Condition¹

Division of Stem Cell Biology,³ Medical Research Center, MizMedi Hospital, 157-280 Seoul, Korea Department of Life Science,⁴ College of Natural Sciences, Hanyang University, 133-791 Seoul, Korea

Human embryonic stem (hES) cells are usually established and maintained on mouse embryonic fibroblast (MEFs) feeder layers. However, it is desirable to develop human feeder cells because animal feeder cells are associated with risks such as viral infection and/or pathogen transmission. In this study, we attempted to establish new hES cell lines using human uterine endometrial cells (hUECs) to prevent the risks associated with animal feeder cells and for their eventual application in cell-replacement therapy. Inner cell masses (ICMs) of cultured blastocysts were isolated by immunosurgery and then cultured on mitotically inactivated hUEC feeder layers. Cultured ICMs formed colonies by continuous proliferation and were allowed to proliferate continuously for 40, 50, and 55 passages. The established hES cell lines (Miz-hES-14, -15, and -9, respectively) exhibited typical hES cells characteristics, including continuous growth, expression of specific markers, normal karyotypes, and differentiation capacity. The hUEC feeders have the advantage that they can be used for many passages, whereas MEF feeder cells can only be used as feeder cells for a limited number of passages. The hUECs are available to establish and maintain hES cells, and the high expression of embryotrophic factors and extracellular matrices by hUECs may be important to the efficient growth of hES cells. Clinical applications require the establishment and expansion of hES cells under stable xeno-free culture systems.

¹ Supported by grants (SC12021 and SC11012) from Stem Cell Research Center of the 21C Frontier R & D Program funded by the Ministry of Science and Technology, Republic of Korea.

² Correspondence: Hyun Soo Yoon, Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, 701-4, Naebalsan-dong, Kangseo-ku, Seoul 157-280, Korea. FAX: 82 22 007 1852; yoon{at}mizmedi.net

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