| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
The application of microarray technology to the study of mammalian organogenesis can provide greater insights into the steps necessary to elicit a functionally competent tissue. To this end, a temporal profile of gene expression was generated with the purpose of identifying changes in gene expression occurring within the developing male and female embryonic gonad. Gonad tissue was collected from mouse embryos at 11.5, 12.5, 14.5, 16.5, and 18.5 days postcoitum (dpc) and relative steady-state levels of mRNA were determined using the Affymetrix MGU74v2 microarray platform. Statistical analysis produced 3693 transcripts exhibiting differential expression during male and/or female gonad development. At 11.5 dpc, the gonad is morphologically indifferent, but at 12.5 dpc, transitions to a male or female phenotype are discernible by the appearance of testicular cords. A number of genes are expressed during this period and many share similar expression profiles in both sexes. As expected, the expression of two well-known sex determination genes, specifically Sry and Sox9, is unique to the testis. Beyond 12.5 dpc, differential gene expression becomes increasingly evident as the male and female tissue morphologically and physiologically diverges. This is evident by two unique waves of transcriptional activity occurring after 14.5 dpc in the male and female. With this study, a large number of transcripts comprising the murine transcriptome can be examined throughout male and female embryonic gonad development and allow for a more complete description of gonad differentiation and development.
2 Correspondence: Michael D. Griswold, 531 Fulmer Hall, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660. FAX: 509 335 9688; mgriswold{at}wsu.edu
3 Current address: Department of Animal Science, Rutgers University, New Brunswick, NJ 08901
This article has been cited by other articles:
|
|
 |
|
  Y. Li, Q. Zhou, R. Hively, L. Yang, C. Small, and M. D. Griswold Differential Gene Expression in the Testes of Different Murine Strains Under Normal and Hyperthermic Conditions J Androl, May 1, 2009; 30(3): 325 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Maywood, S. Chahad-Ehlers, M. L. Garabette, C. Pritchard, P. Underhill, A. Greenfield, F. J. P. Ebling, R. A. Akhtar, C. P. Kyriacou, M. H. Hastings, et al. Differential Testicular Gene Expression in Seasonal Fertility J Biol Rhythms, April 1, 2009; 24(2): 114 - 125. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Feng, X. Peng, S. Li, and Y. Gong Isolation and characterization of sexual dimorphism genes expressed in chicken embryonic gonads Acta Biochim Biophys Sin, April 1, 2009; 41(4): 285 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Fowler, S. Flannigan, A. Mathers, K. Gillanders, R. G. Lea, M. J. Wood, A. Maheshwari, S. Bhattacharya, E. S. R. Collie-Duguid, P. J. Baker, et al. Gene Expression Analysis of Human Fetal Ovarian Primordial Follicle Formation J. Clin. Endocrinol. Metab., April 1, 2009; 94(4): 1427 - 1435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Beverdam, T. Svingen, S. Bagheri-Fam, P. Bernard, P. McClive, M. Robson, M. B. Khojasteh, M. Salehi, A. H Sinclair, V. R Harley, et al. Sox9-dependent expression of Gstm6 in Sertoli cells during testis development in mice Reproduction, March 1, 2009; 137(3): 481 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K L Hahn, B Beres, M. J Rowton, M K Skinner, Y Chang, A Rawls, and J Wilson-Rawls A deficiency of lunatic fringe is associated with cystic dilation of the rete testis Reproduction, January 1, 2009; 137(1): 79 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Barakat, A. E O'Connor, E. Gold, D. M de Kretser, and K. L Loveland Inhibin, activin, follistatin and FSH serum levels and testicular production are highly modulated during the first spermatogenic wave in mice Reproduction, September 1, 2008; 136(3): 345 - 359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ikeda, H. Tanaka, and M. Esaki Effects of Gestational Diethylstilbestrol Treatment on Male and Female Gonads during Early Embryonic Development Endocrinology, August 1, 2008; 149(8): 3970 - 3979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhou, R. Nie, Y. Li, P. Friel, D. Mitchell, R. A Hess, C. Small, and M. D Griswold Expression of Stimulated by Retinoic Acid Gene 8 (Stra8) in Spermatogenic Cells Induced by Retinoic Acid: An In Vivo Study in Vitamin A-Sufficient Postnatal Murine Testes Biol Reprod, July 1, 2008; 79(1): 35 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Coveney, J. Cool, T. Oliver, and B. Capel From the Cover: Four-dimensional analysis of vascularization during primary development of an organ, the gonad PNAS, May 20, 2008; 105(20): 7212 - 7217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Qing, H. Liu, W. Wei, X. Ye, W. Shen, D. Zhang, Z. Song, W. Yang, M. Ding, and H. Deng Mature oocytes derived from purified mouse fetal germ cells Hum. Reprod., January 1, 2008; 23(1): 54 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. O'Shaughnessy, P. J. Baker, A. Monteiro, S. Cassie, S. Bhattacharya, and P. A. Fowler Developmental Changes in Human Fetal Testicular Cell Numbers and Messenger Ribonucleic Acid Levels during the Second Trimester J. Clin. Endocrinol. Metab., December 1, 2007; 92(12): 4792 - 4801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M Clement, M. D Anway, M. Uzumcu, and M. K Skinner Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes Reproduction, September 1, 2007; 134(3): 455 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Gallardo, G. B. John, L. Shirley, C. M. Contreras, E. A. Akbay, J. M. Haynie, S. E. Ward, M. J. Shidler, and D. H. Castrillon Genomewide Discovery and Classification of Candidate Ovarian Fertility Genes in the Mouse Genetics, September 1, 2007; 177(1): 179 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Reynard, J. M.A. Turner, J. Cocquet, S. K. Mahadevaiah, A. Toure, C. Hoog, and P. S. Burgoyne Expression Analysis of the Mouse Multi-Copy X-Linked Gene Xlr-Related, Meiosis-Regulated (Xmr), Reveals That Xmr Encodes a Spermatid-Expressed Cytoplasmic Protein, SLX/XMR Biol Reprod, August 1, 2007; 77(2): 329 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Santos, V. L. Workman, G. C. Paull, A. L. Filby, K. J. W. Van Look, P. Kille, and C. R. Tyler Molecular basis of sex and reproductive status in breeding zebrafish Physiol Genomics, July 18, 2007; 30(2): 111 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wilhelm, R. Hiramatsu, H. Mizusaki, L. Widjaja, A. N. Combes, Y. Kanai, and P. Koopman SOX9 Regulates Prostaglandin D Synthase Gene Transcription in Vivo to Ensure Testis Development J. Biol. Chem., April 6, 2007; 282(14): 10553 - 10560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Xia, D. D Mruk, W. M Lee, and C Y. Cheng Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model J. Endocrinol., March 1, 2007; 192(3): 563 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Itman, S. Mendis, B. Barakat, and K. L. Loveland All in the family: TGF-{beta} family action in testis development. Reproduction, August 1, 2006; 132(2): 233 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Lee, C. P. Houk, S. F. Ahmed, I. A. Hughes, and in collaboration with the participants in the Inte Consensus Statement on Management of Intersex Disorders Pediatrics, August 1, 2006; 118(2): e488 - e500. [Full Text] [PDF]
|
|
|
|
|
|
J. A. Schmidt, J. M. de Avila, and D. J. McLean Grafting Period and Donor Age Affect the Potential for Spermatogenesis in Bovine Ectopic Testis Xenografts Biol Reprod, August 1, 2006; 75(2): 160 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I A Hughes, C Houk, S F Ahmed, P A Lee, and LWPES/ESPE Consensus Group Consensus statement on management of intersex disorders Arch. Dis. Child., July 1, 2006; 91(7): 554 - 563. [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. David Proposed Mode of Action for In Utero Effects of Some Phthalate Esters on the Developing Male Reproductive Tract Toxicol Pathol, April 1, 2006; 34(3): 209 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Beverdam and P. Koopman Expression profiling of purified mouse gonadal somatic cells during the critical time window of sex determination reveals novel candidate genes for human sexual dysgenesis syndromes Hum. Mol. Genet., February 1, 2006; 15(3): 417 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Robert, L. J. Martin, and J. J. Tremblay The Orphan Nuclear Receptor NR4A1 Regulates Insulin-Like 3 Gene Transcription in Leydig Cells Biol Reprod, February 1, 2006; 74(2): 322 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Reynolds, B. Collier, K. Maratou, V. Bingham, R. M. Speed, M. Taggart, C. A. Semple, N. K. Gray, and H. J. Cooke Dazl binds in vivo to specific transcripts and can regulate the pre-meiotic translation of Mvh in germ cells Hum. Mol. Genet., December 15, 2005; 14(24): 3899 - 3909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Wagoner, G Sanchez, A-N Nguyen, G C Enders, and G Blanco Different expression and activity of the {alpha}1 and {alpha}4 isoforms of the Na,K-ATPase during rat male germ cell ontogeny Reproduction, November 1, 2005; 130(5): 627 - 641. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
