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Centre for Biomedical Research,3 University of Victoria, Victoria, British Columbia, Canada V8W 3N5
Great Lakes Water Institute,4 University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53204
Simon Fraser University,5 Burnaby, British Columbia, Canada V5A 1S6
Development and maturation of the ovary requires precisely coordinated expression of specific gene classes to produce viable oocytes. We undertook identification of some of the genes involved in these processes by creating ovary-specific cDNA libraries by suppression subtractive hybridization and by microarray-based analyses. We present 5778 tissue- and sex-specific genes from subtracted ovary and testis libraries, many of which remain unidentified. A microarray containing 3557 salmonid cDNAs was used to compare the transcriptomes of precocious ovary at three different stages during the second year of life with a reference (normal ovary) transcriptome. On average, approximately 240 genes were developmentally regulated during the study period from June to October. Classes of genes maintaining relatively steady-state levels of expression, such as those controlling tissue remodeling, immunoregulation, cell-cycle progression, apoptosis, and growth also were identified. Concurrent expression of various cell division and ubiquitin-mediated proteolysis regulators revealed the utility of microarray analysis to monitor important maturation events. We also report unequivocal evidence for expression of the transcripts that encode the common glycoprotein 
, LHß, FSHß, thyroid-stimulating hormone ß, and retinol-binding protein in both the ovary and testis of trout.
2 Correspondence: Ben F. Koop, Centre for Biomedical Research, University of Victoria, P.O. Box 3020 STN CSC, Victoria, British Columbia V8W 3N5, Canada. FAX: 250 472 4075; bkoop{at}uvic.ca
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