| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Department of Histology and Embryology Faculty of Medicine,3 Akdeniz University, Antalya 07070, Turkey
Department of Obstetrics and Gynecology,4 Yale University, School of Medicine, New Haven, Connecticut 06520-8063
Placental vasculogenesis consists of several stages, including appearance of hemangioblasts and angiogenic cell islands, setting up a primitive vascular network, and transition from vasculogenesis to sprouting and nonsprouting angiogenesis. In the present study, we hypothesized that placental vasculogenesis and angiogenesis require apoptosis during the formation of primitive vascular pattern, vessel elongation, and angiogenic branching. Vasculogenesis and apoptotic cells were identified using CD31 immunohistochemistry, hematoxylin-eosin (H-E) staining, CD31-TUNEL double-labeling, and transmission-electron microscopy (TEM). No TUNEL-positive cell was detected in angiogenic cell islands; however, several TUNEL-positive cells were observed during the primitive lumen formation. Interestingly, some of the stromal cells located between vasculogenic areas during the endothelial tube elongation and angiogenic branching also were TUNEL-positive. The presence of morphological aspects of apoptosis, such as nuclear shrinkage and nuclear bodies (apoptotic bodies), also was confirmed in H-E-stained and TEM-depicted sections. Quantitative analysis showed that higher ratios for apoptotic cells were found in the core stroma of villi among the vascular branching areas and in the primitive capillary lumen compared to angiogenic cell cords and vasculatures with advanced lumens (P < 0.05). In conclusion, our results suggest that apoptosis likely is involved in the physiologic mechanisms of placental vasculogenesis and angiogenesis, such as lumen formation and angiogenic branching.
2 Correspondence: Umit A. Kayisli, Department of Histology and Embryology Faculty of Medicine, Akdeniz University, Antalya 07070, Turkey. FAX: 90 242 227 4486; uali{at}akdeniz.edu.tr
This article has been cited by other articles:
|
|
 |
|
  J. Niu, A. Azfer, O. Zhelyabovska, S. Fatma, and P. E. Kolattukudy Monocyte Chemotactic Protein (MCP)-1 Promotes Angiogenesis via a Novel Transcription Factor, MCP-1-induced Protein (MCPIP) J. Biol. Chem., May 23, 2008; 283(21): 14542 - 14551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Soupene, V. Serikov, and F. A. Kuypers Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells J. Lipid Res., May 1, 2008; 49(5): 1103 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.A.M. Lisman, M.J.B. van den Hoff, K. Boer, O.P. Bleker, K. van Groningen, and N. Exalto The architecture of first trimester chorionic villous vascularization: a confocal laser scanning microscopical study Hum. Reprod., August 1, 2007; 22(8): 2254 - 2260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. G Clarke, S. A Hope, S. Byers, and R. J Rodgers Formation of ovarian follicular fluid may be due to the osmotic potential of large glycosaminoglycans and proteoglycans Reproduction, July 1, 2006; 132(1): 119 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Kunz-Schughart, J. A. Schroeder, M. Wondrak, F. van Rey, K. Lehle, F. Hofstaedter, and D. N. Wheatley Potential of fibroblasts to regulate the formation of three-dimensional vessel-like structures from endothelial cells in vitro Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1385 - C1398. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
