HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 72/4/932    most recent biolreprod.104.035105v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wakayama, S. Articles by Wakayama, T. Search for Related Content PubMed PubMed Citation Articles by Wakayama, S. Articles by Wakayama, T. Agricola Articles by Wakayama, S. Articles by Wakayama, T.

Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell Lines from Different Mouse Strains and Tissues¹

Center for Developmental Biology,³ RIKEN Kobe, Kobe 650-0047, Japan Department of Life Science,⁴ Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan Graduate School of Agricultural Science,⁵ Tohoku University, Sendai 981-8555, Japan

Nuclear transfer can be used to generate embryonic stem cell lines from somatic cells, and these have great potential in regenerative medicine. However, it is still unclear whether any individual or cell type can be used to generate such lines. Here, we tested seven different male and female mouse genotypes and three cell types as sources of nuclei to determine the efficiency of establishing nuclear transfer embryonic stem cell lines. Lines were successfully established from all sources. Cumulus cell nuclei from F₁ mouse genotypes showed a significantly higher cumulative establishment rate from reconstructed oocytes than from other cells; however, there were no genotype differences in success rates from cloned blastocysts. Thus, the overall success depends on preimplantation development, and, once embryos have reached the blastocyst stage, the genotype differences disappear. All mouse genotypes that were tested demonstrated at least one cell line that subsequently contributed to germline transmission in chimeric mice, so these cell lines clearly possess the same potential as embryonic stem cells derived from fertilized embryos. Thus, nuclear transfer embryonic stem cells can be generated relatively easily from a variety of inbred mouse genotypes and cell types of both sexes, even though it may be more difficult to generate clones directly.

¹ Supported by a Grant-in-Aid for Creative Scientific Research (13GS0008), Scientific Research in Priority Areas (15080211), Young Scientists A (15681014), and a project for the realization of regenerative medicine (the research field for the technical development of stem cell manipulation) to T.W. from the Ministry of Education, Science, Sports, Culture and Technology of Japan.

² Correspondence: Teruhiko Wakayama, 2-2-3 Minatojima-minamimachi Chuo-ku, Kobe 650-0047, Japan. FAX: 81 78 306 0101;teru{at}cdb.riken.go.jp

This article has been cited by other articles:

				S. Wakayama, H. Ohta, T. Hikichi, E. Mizutani, T. Iwaki, O. Kanagawa, and T. Wakayama Production of healthy cloned mice from bodies frozen at -20{degrees}C for 16 years PNAS, November 11, 2008; 105(45): 17318 - 17322. [Abstract] [Full Text] [PDF]

				H. Ohta, Y. Sakaide, and T. Wakayama Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages Reproduction, November 1, 2008; 136(5): 581 - 587. [Abstract] [Full Text] [PDF]

				H. Ohta, Y. Sakaide, K. Yamagata, and T. Wakayama Increasing the Cell Number of Host Tetraploid Embryos Can Improve the Production of Mice Derived from Embryonic Stem Cells Biol Reprod, September 1, 2008; 79(3): 486 - 492. [Abstract] [Full Text] [PDF]

				B. Heindryckx, P. De Sutter, J. Gerris, M. Dhont, and J. Van der Elst Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes Hum. Reprod., July 1, 2007; 22(7): 1982 - 1990. [Abstract] [Full Text] [PDF]

				E. Mizutani, H. Ohta, S. Kishigami, N. Van Thuan, T. Hikichi, S. Wakayama, M. Kosaka, E. Sato, and T. Wakayama Developmental ability of cloned embryos from neural stem cells. Reproduction, December 1, 2006; 132(6): 849 - 857. [Abstract] [Full Text] [PDF]

				R. Alberio, K. H Campbell, and A. D Johnson Reprogramming somatic cells into stem cells. Reproduction, November 1, 2006; 132(5): 709 - 720. [Abstract] [Full Text] [PDF]

				S. Wakayama, M. L. Jakt, M. Suzuki, R. Araki, T. Hikichi, S. Kishigami, H. Ohta, N. Van Thuan, E. Mizutani, Y. Sakaide, et al. Equivalency of Nuclear Transfer-Derived Embryonic Stem Cells to Those Derived from Fertilized Mouse Blastocysts Stem Cells, September 1, 2006; 24(9): 2023 - 2033. [Abstract] [Full Text] [PDF]

				R. Blelloch, Z. Wang, A. Meissner, S. Pollard, A. Smith, and R. Jaenisch Reprogramming Efficiency Following Somatic Cell Nuclear Transfer Is Influenced by the Differentiation and Methylation State of the Donor Nucleus Stem Cells, September 1, 2006; 24(9): 2007 - 2013. [Abstract] [Full Text] [PDF]

				X.-M. Guo, Y.-S. Zhao, H.-X. Chang, C.-Y. Wang, L.-L. E, X.-A. Zhang, C.-M. Duan, L.-Z. Dong, H. Jiang, J. Li, et al. Creation of Engineered Cardiac Tissue In Vitro From Mouse Embryonic Stem Cells Circulation, May 9, 2006; 113(18): 2229 - 2237. [Abstract] [Full Text] [PDF]

				S. L. Smith, R. E. Everts, X. C. Tian, F. Du, L.-Y. Sung, S. L. Rodriguez-Zas, B.-S. Jeong, J.-P. Renard, H. A. Lewin, and X. Yang Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning PNAS, December 6, 2005; 102(49): 17582 - 17587. [Abstract] [Full Text] [PDF]