HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 72/5/1169    most recent biolreprod.104.032953v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liptak, A. R. Articles by Townson, D. H. Search for Related Content PubMed PubMed Citation Articles by Liptak, A. R. Articles by Townson, D. H. Agricola Articles by Liptak, A. R. Articles by Townson, D. H.

Cooperative Expression of Monocyte Chemoattractant Protein 1 Within the Bovine Corpus Luteum: Evidence of Immune Cell-Endothelial Cell Interactions in a Coculture System¹

Department of Animal and Nutritional Sciences,³ University of New Hampshire, Durham, New Hampshire 03824 Vincent Center for Reproductive Biology,⁴ Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114 Department of Animal Science,⁵ University of Peradeniya, Peradeniya 20400, Sri Lanka Department of Agricultural and Life Science,⁶ Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan Department of Obstetrics and Gynecology,⁷ University Nebraska Medical Center and Veterans Affairs Medical Center, Omaha, Nebraska 68198

Endothelial cells (EC) of the bovine corpus luteum (CL) are a known source of proinflammatory mediators, including monocyte chemoattractant protein 1 (CCL2) and endothelin 1 (EDN1). Here, a coculture system was devised to determine if immune cells and PGF₂ together affect CCL2 and EDN1 secretion by EC. Luteal EC were cultured either alone or together with peripheral blood mononuclear cells (PBMC), and treated without or with PGF₂ for 48 h (n = 6 experiments). Coculture of EC with PBMC increased CCL2 secretion an average of 5-fold higher compared with either cell type alone (P < 0.05). Basal secretion of EDN1 by EC was substantial (2 ng/ml), but was not affected by coculture with PBMC (P > 0.05). EC cocultured with concanavalin A-activated PBMC (ActPBMC) increased CCL2 secretion an average of 12-fold higher compared with controls (P < 0.05), but again, EDN1 secretion was unchanged (P > 0.05). Interestingly, PGF₂ did not alter either CCL2 or EDN1 secretion, regardless of culture conditions (P > 0.05). In a second series of experiments (n = 3 experiments), mixed luteal cells (MLC) were cultured alone or with PBMC as described above. Secretion of CCL2 and EDN1 was not affected by coculture or by PGF₂ (P > 0.05), but MLC produced less progesterone in the presence of ActPBMC (P < 0.05). Collectively, these results suggest that immune cells and EC can interact cooperatively to increase CCL2 secretion in the CL, but this interaction does not affect EDN1 secretion nor is it influenced by PGF₂. Additionally, activated immune cells appear to produce a factor that impairs progesterone production by luteal steroidogenic cells.

corpus luteum, corpus luteum function, cytokines, immunology, progesterone

² Correspondence: David Townson, 509 Kendall Hall, Durham, NH 03824-3590 FAX: 603 862 3758; dave.townson{at}unh.edu

This article has been cited by other articles:

				A. Bowolaksono, R. Nishimura, T. Hojo, R. Sakumoto, T. J. Acosta, and K. Okuda Anti-Apoptotic Roles of Prostaglandin E2 and F2alpha in Bovine Luteal Steroidogenic Cells Biol Reprod, August 1, 2008; 79(2): 310 - 317. [Abstract] [Full Text] [PDF]

				L. E. Henkes, B. T. Sullivan, M. P. Lynch, R. Kolesnick, D. Arsenault, M. Puder, J. S. Davis, and B. R. Rueda Acid sphingomyelinase involvement in tumor necrosis factor {alpha}-regulated vascular and steroid disruption during luteolysis in vivo PNAS, June 3, 2008; 105(22): 7670 - 7675. [Abstract] [Full Text] [PDF]

				K. Shirasuna, S. Watanabe, T. Asahi, M. P B Wijayagunawardane, K. Sasahara, C. Jiang, M. Matsui, M. Sasaki, T. Shimizu, J. S Davis, et al. Prostaglandin F2{alpha} increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum: the possible regulation of blood flow at an early stage of luteolysis Reproduction, April 1, 2008; 135(4): 527 - 539. [Abstract] [Full Text] [PDF]

				M. J Cannon, J. S Davis, and J. L Pate The class II major histocompatibility complex molecule BoLA-DR is expressed by endothelial cells of the bovine corpus luteum Reproduction, May 1, 2007; 133(5): 991 - 1003. [Abstract] [Full Text] [PDF]

				D. H. Townson Immune cell-endothelial cell interactions in the bovine corpus luteum Integr. Comp. Biol., December 1, 2006; 46(6): 1055 - 1059. [Abstract] [Full Text] [PDF]

				M. J Cannon, J. S Davis, and J. L Pate Presence and regulation of messenger ribonucleic acids encoding components of the class II major histocompatibility complex-associated antigen processing pathway in the bovine corpus luteum. Reproduction, April 1, 2006; 131(4): 689 - 698. [Abstract] [Full Text] [PDF]