HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 73/2/324    most recent biolreprod.104.038026v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ndiaye, K. Articles by Lussier, J. G. Search for Related Content PubMed PubMed Citation Articles by Ndiaye, K. Articles by Lussier, J. G. Agricola Articles by Ndiaye, K. Articles by Lussier, J. G.

Identification of Downregulated Messenger RNAs in Bovine Granulosa Cells of Dominant Follicles Following Stimulation with Human Chorionic Gonadotropin¹

Centre de recherche en reproduction animale, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 7C6

Molecular determinants and mechanisms involved in ovarian follicular growth, ovulation, and luteinization are not well understood. The objective of this study was to identify genes expressed in bovine granulosa cells (GC) of dominant follicles (DF) and downregulated after hCG-induced ovulation, using the suppression subtractive hybridization (SSH). GC were collected from DF at Day 5 of the estrous cycle and from ovulatory follicles (OF) obtained 23 h following injection of hCG. A subtracted cDNA library (DF-OF) was generated and screened using unsubtracted (DF, OF) and subtracted (DF-OF, OF-DF) cDNAs as complex ³²P-probes. A total of 32 nonredundant cDNAs were identified: 23 cDNAs matched with sequences of known biological function and 9 cDNAs with complete or partial sequences of undefined biological function. Detection of genes known to be downregulated during the periovulatory period in the bovine species, such as CPD, CYP11A1, CYP19A1, FSHR, LRP8/ ApoER2, and SERPINE2, validated the physiological model and analytical techniques used. For a subset of genes, such as ARFGAP3, CYP11A1, CYP19A1, FSHR, FST, GJA1, IDH3, INHBA, LHCGR, LHCGR lacking exon 10, PRC1, PRG1, RPA2, SCD, and TRIB2, gene expression profiles were compared by virtual Northern blot or reverse transcriptase-polymerase chain reaction from follicles obtained at different developmental stages. Results confirmed a downregulation of the respective mRNAs in GC of OF compared with that of DF. We conclude that we have identified novel genes that are downregulated by hCG in bovine GC of DF during the periovulatory period, which may contribute to follicular growth, ovulation, and/or luteinization.

dominance, follicle, gene expression, gene regulation, granulosa cells, ovary, ovulation

² Correspondence: Jacques G. Lussier, Faculté de médecine vétérinaire, Université de Montréal, P.O. Box 5000, St-Hyacinthe, Québec, Canada, J2S 7C6. FAX: 450 778 8103; jacques.lussier{at}umontreal.ca

This article has been cited by other articles:

				M. Hamel, I. Dufort, C. Robert, C. Gravel, M.-C. Leveille, A. Leader, and M.-A. Sirard Identification of differentially expressed markers in human follicular cells associated with competent oocytes Hum. Reprod., May 1, 2008; 23(5): 1118 - 1127. [Abstract] [Full Text] [PDF]

				A Bonnet, R Dalbies-Tran, and M A Sirard Opportunities and challenges in applying genomics to the study of oogenesis and folliculogenesis in farm animals Reproduction, February 1, 2008; 135(2): 119 - 128. [Abstract] [Full Text] [PDF]

				H. L Henderson, D. J Hodson, S. J Gregory, J. Townsend, and D. J Tortonese Gonadotropin-Releasing Hormone Stimulates Prolactin Release from Lactotrophs in Photoperiodic Species Through a Gonadotropin-Independent Mechanism Biol Reprod, February 1, 2008; 78(2): 370 - 377. [Abstract] [Full Text] [PDF]

				O.J Ginther, M.D Utt, M.A Beg, E.L Gastal, and M.O Gastal Negative Effect of Estradiol on Luteinizing Hormone Throughout the Ovulatory Luteinizing Hormone Surge in Mares Biol Reprod, September 1, 2007; 77(3): 543 - 550. [Abstract] [Full Text] [PDF]

				T. Fayad, R. Lefebvre, J. Nimpf, D. W. Silversides, and J. G. Lussier Low-Density Lipoprotein Receptor-Related Protein 8 (LRP8) Is Upregulated in Granulosa Cells of Bovine Dominant Follicle: Molecular Characterization and Spatio-Temporal Expression Studies Biol Reprod, March 1, 2007; 76(3): 466 - 475. [Abstract] [Full Text] [PDF]

				C. Agca, J. E Ries, S. J Kolath, J.-H. Kim, L. J Forrester, E. Antoniou, K. M Whitworth, N. Mathialagan, G. K Springer, R. S Prather, et al. Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression Reproduction, July 1, 2006; 132(1): 133 - 145. [Abstract] [Full Text] [PDF]

				M. N. Diouf, K. Sayasith, R. Lefebvre, D. W. Silversides, J. Sirois, and J. G. Lussier Expression of Phospholipase A2 Group IVA (PLA2G4A) Is Upregulated by Human Chorionic Gonadotropin in Bovine Granulosa Cells of Ovulatory Follicles Biol Reprod, June 1, 2006; 74(6): 1096 - 1103. [Abstract] [Full Text] [PDF]

				E L Gastal, M O Gastal, and O J Ginther Relationships of changes in B-mode echotexture and colour-Doppler signals in the wall of the preovulatory follicle to changes in systemic oestradiol concentrations and the effects of human chorionic gonadotrophin in mares. Reproduction, April 1, 2006; 131(4): 699 - 709. [Abstract] [Full Text] [PDF]

				M. Cao, J. Buratini Jr, J. G Lussier, P. D Carriere, and C. A Price Expression of protease nexin-1 and plasminogen activators during follicular growth and the periovulatory period in cattle Reproduction, January 1, 2006; 131(1): 125 - 137. [Abstract] [Full Text] [PDF]