Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print April 27, 2005.
Biol Reprod 2005, 10.1095/biolreprod.105.040337
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
73/2/374    most recent
biolreprod.105.040337v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lash, G. E.
Right arrow Articles by Robson, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lash, G. E.
Right arrow Articles by Robson, S. C.
Agricola
Right arrow Articles by Lash, G. E.
Right arrow Articles by Robson, S. C.
BIOLOGY OF REPRODUCTION 73, 374–381 (2005)
DOI: 10.1095/biolreprod.105.040337
© 2005 by the Society for the Study of Reproduction, Inc.

Inhibition of Trophoblast Cell Invasion by TGFB1, 2, and 3 Is Associated with a Decrease in Active Proteases1

Gendie E. Lash 2 3, Harry A. Otun 3, Barbara A. Innes 4, Judith N. Bulmer 4, Roger F. Searle 5, and Stephen C. Robson 3

Schools of Surgical and Reproductive Sciences,3 Clinical and Laboratory Sciences,4 Medical Education Development,5 University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom

Invasion of extravillous trophoblast cells into the uterus in human pregnancy is tightly regulated. The transforming growth factor-beta (TGFB) family has been suggested to play a role in controlling this process. We hypothesized that TGFB1, 2, and 3 would inhibit the invasive capacity of extravillous trophoblast cells. We also studied trophoblast apoptosis and proliferation and secreted protease levels as potential mechanisms by which these cytokines may act. Inhibition of endogenous TGFB1, 2, and 3 with neutralizing antibodies increased the invasive capacity of extravillous trophoblast cells derived from placental explants. Similarly, addition of exogenous TGFB1, 2, and 3 inhibited the invasive capacity of these cells in a dose-dependent manner. Proliferation of trophoblast in the placental explants did not alter in response to any of the cytokines tested. Apoptosis of villous and extravillous trophoblast did not alter in response to TGFB1, 2, and 3. There was a reduction in secreted levels of matrix metalloproteinase (MMP) 9 and urokinase plasminogen activator in response to all three cytokines. MMP2 and tissue inhibitor of metalloproteinase 1 and 3 levels were not altered. These results suggest that TGFB1, 2, and 3 inhibit trophoblast invasion by a mechanism dependent on reduced protease activity.

cytokines, implantation, placenta, pregnancy, trophoblast

1 Supported by funding from BBSRC (S19967).

2 Correspondence: Gendie Lash, School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom. FAX: 44 191 222 5066; g.e.lash{at}ncl.ac.uk




This article has been cited by other articles:


Home page
 Hum ReprodHome page
K. K.W. Lam, P. C.N. Chiu, M.-K. Chung, C.-L. Lee, K.-F. Lee, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, and W. S.B. Yeung
Glycodelin-A as a modulator of trophoblast invasion
Hum. Reprod., June 11, 2009; (2009) dep205v1.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. Naruse, G. E. Lash, B. A. Innes, H. A. Otun, R. F. Searle, S. C. Robson, and J. N. Bulmer
Localization of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitors for MMPs (TIMPs) in uterine natural killer cells in early human pregnancy
Hum. Reprod., March 1, 2009; 24(3): 553 - 561.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
S. M. Nelson and I. A. Greer
The potential role of heparin in assisted conception
Hum. Reprod. Update, November 1, 2008; 14(6): 623 - 645.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. W. Bonagura, G. J. Pepe, A. C. Enders, and E. D. Albrecht
Suppression of Extravillous Trophoblast Vascular Endothelial Growth Factor Expression and Uterine Spiral Artery Invasion by Estrogen during Early Baboon Pregnancy
Endocrinology, October 1, 2008; 149(10): 5078 - 5087.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Fafet, C. Rebouissou, T. Maudelonde, and M.-L. Vignais
Opposite Effects of Transforming Growth Factor-{beta} Activation and Rho-Associated Kinase Inhibition on Human Trophoblast Migration in a Reconstituted Placental-Endometrial Coculture System
Endocrinology, September 1, 2008; 149(9): 4475 - 4485.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
G. E. Lash, H. A. Otun, B. A. Innes, M. Kirkley, L. De Oliveira, R. F. Searle, S. C. Robson, and J. N. Bulmer
Interferon-{gamma} inhibits extravillous trophoblast cell invasion by a mechanism that involves both changes in apoptosis and protease levels
FASEB J, December 1, 2006; 20(14): 2512 - 2518.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. J. Scaife, J. N. Bulmer, S. C. Robson, B. A. Innes, and R. F. Searle
Effector Activity of Decidual CD8+ T Lymphocytes in Early Human Pregnancy
Biol Reprod, October 1, 2006; 75(4): 562 - 567.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M.-r. Zhao, W. Qiu, Y.-x. Li, Z.-b. Zhang, D. Li, and Y.-l. Wang
Dual effect of transforming growth factor {beta}1 on cell adhesion and invasion in human placenta trophoblast cells.
Reproduction, August 1, 2006; 132(2): 333 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
G. Nie, Y. Li, K. Hale, H. Okada, U. Manuelpillai, E. M. Wallace, and L. A. Salamonsen
Serine Peptidase HTRA3 Is Closely Associated with Human Placental Development and Is Elevated in Pregnancy Serum
Biol Reprod, February 1, 2006; 74(2): 366 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
G. E. Lash, H. A. Otun, B. A. Innes, J. N. Bulmer, R. F. Searle, and S. C. Robson
Low Oxygen Concentrations Inhibit Trophoblast Cell Invasion from Early Gestation Placental Explants via Alterations in Levels of the Urokinase Plasminogen Activator System
Biol Reprod, February 1, 2006; 74(2): 403 - 409.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for the Study of Reproduction.