Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print May 11, 2005.
Biol Reprod 2005, 10.1095/biolreprod.105.041152
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
73/3/443    most recent
biolreprod.105.041152v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Related articles in Biol Reprod
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gieseke, C.
Right arrow Articles by Talbot, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gieseke, C.
Right arrow Articles by Talbot, P.
Agricola
Right arrow Articles by Gieseke, C.
Right arrow Articles by Talbot, P.
BIOLOGY OF REPRODUCTION 73, 443–451 (2005)
DOI: 10.1095/biolreprod.105.041152
© 2005 by the Society for the Study of Reproduction, Inc.

Cigarette Smoke Inhibits Hamster Oocyte Pickup by Increasing Adhesion Between the Oocyte Cumulus Complex and Oviductal Cilia1

Christine Gieseke , and Prue Talbot 2 

Department of Cell Biology and Neuroscience, University of California, Riverside, California 92521

Previous research has shown that oocyte cumulus complex (OCC) pickup rate is inhibited in hamsters when oviducts and OCCs are simultaneously exposed to cigarette smoke solutions, independent of any effect on ciliary beat frequency. The purpose of this research was to determine whether smoke solutions caused a change in adhesion between the OCC and infundibulum of the oviduct and to determine whether a change in adhesion could account for decreased OCC pickup rate. OCC pickup rate and adhesion were measured before and after infundibula or OCCs recovered from acute in vitro exposures to mainstream and sidestream whole, gas, or particulate smoke solutions. Ciliary beat frequency was also measured on infundibula. Overall, smoke solutions decreased oocyte pickup rate 40% to 80% below control levels and increased adhesion 52% to 91% above control levels when infundibula were pretreated. A change in adhesion was observed in cases for which decreased OCC pickup rate could not be explained by a change in ciliary beat frequency. OCC pickup rate decreased 20% to 35% below control levels and adhesion increased 39% to 54% above control levels when OCCs were pretreated. These data show not only that cigarette smoke causes an increase in OCC adhesion to the oviduct, but also that there is a correlation between increased adhesion and decreased OCC pickup rate. The results also show that the both the OCC and oviduct are targets of cigarette smoke. The oviduct is more sensitive to the adverse effects of smoke; however, this may be caused by a combined impact on mechanisms involved in both adhesion and ciliary function.

cumulus cells, female reproductive tract, oviduct, ovum pickup/ transport, toxicology

1 Supported by Tobacco-Related Disease Research Program 10RT-029 and 13RT-0068.

2 Correspondence: Prue Talbot, Department of Cell Biology and Neuroscience, University of California, Riverside CA 92521. FAX: 951-827-4286; talbot{at}ucr.edu


Related articles in Biol Reprod:

Highlights
Mary Ann Handel and John Eppig
Biol Reprod 2005 73: 389. [Full Text]  





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for the Study of Reproduction.