Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print May 18, 2005.
Biol Reprod 2005, 10.1095/biolreprod.104.038331
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
73/3/565    most recent
biolreprod.104.038331v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cao, W.-G.
Right arrow Articles by Akoum, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cao, W.-G.
Right arrow Articles by Akoum, A.
Agricola
Right arrow Articles by Cao, W.-G.
Right arrow Articles by Akoum, A.
BIOLOGY OF REPRODUCTION 73, 565–570 (2005)
DOI: 10.1095/biolreprod.104.038331
© 2005 by the Society for the Study of Reproduction, Inc.

Stimulation of Macrophage Migration Inhibitory Factor Expression in Endometrial Stromal Cells by Interleukin 1, beta Involving the Nuclear Transcription Factor NF{kappa}B1

W.-G. Cao 3, M. Morin 3, C. Metz 4, R. Maheux 3, and A. Akoum 2, 3

Unité d'endocrinologie de la reproduction,3 Centre de Recherche, Hôpital Saint-François d'Assise, Centre Hospitalier Universitaire de Québec, Faculté de Médecine, Université Laval, Québec, Canada G1L 3L5 Institute for Medical Research at North Shore-LIJ,4 Manhasset, New York 11030

Endometriosis, the ectopic development of endometrial tissue, is, particularly in peritoneal endometriosis, believed to result from tubal reflux of menstrual tissue. The release of cytokines and growth factors by refluxed endometrial cells in response to peritoneal inflammatory stimuli may enhance the capability of endometrial cells to implant and grow into the peritoneal host tissue. Herein we report that interleukin 1 (IL1), a major proinflammatory cytokine that is overproduced by endometriosis women-derived peritoneal macrophages and found in elevated concentrations in the peritoneal fluid of patients with endometriosis, stimulates the synthesis and the secretion of macrophage migration inhibitory factor (MIF) by human endometrial stromal cells. IL1B (0.1–100 ng/ml) exerted dose- and time-dependent effects of MIF protein secretion and mRNA synthesis, as shown by ELISA and reverse transcription-polymerase chain reaction, respectively. IL1B appeared to induce MIF gene transcription via the {kappa}B nuclear transcription factor (NF{kappa}B), as shown by electrophoretic mobility shift assay and Western blot analysis of I{kappa}B phosphorylation. Curcumin (10–8 M), which is known for inhibiting NF{kappa}B activation, inhibited IL1B-induced MIF secretion as well as NF{kappa}B nuclear translocation and DNA binding. Taken together, these findings clearly show that IL1B up-regulates the expression of MIF in endometrial stromal cells in vitro and acts via NF{kappa}B. This may play an important role in the physiology of the human endometrium and the pathophysiology of endometriosis considering the immunomodulatory properties of MIF as well as its role in cell growth, angiogenesis and tissue remodeling.

cytokines, endometrial cells, endometriosis, female reproductive tract, IL1B, menstrual cycle, MIF, NF{kappa}B, uterus

1 Supported by grant MOP-37921 to A.A. from The Canadian Institutes for Health Research. A.A. is Chercheur National from the Fonds de la Recherche en Santé du Québec (FRSQ). W.-G.C. is a recipient of a Wyeth-Ayerst CIHR/Rx&D Fellowship.

2 Correspondence: Ali Akoum, Laboratoire d'Endocrinologie de la Reproduction, Centre de Recherche, Hôpital Saint-François d'Assise, 10 rue de l'Espinay, Local D0-711, Québec, Québec, Canada, G1L 3L5. FAX: 418 525 4195; ali.akoum{at}crsfa.ulaval.ca




This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
F. Wieser, M. Cohen, A. Gaeddert, J. Yu, C. Burks-Wicks, S. L. Berga, and R. N. Taylor
Evolution of medical treatment for endometriosis: back to the roots?
Hum. Reprod. Update, September 1, 2007; 13(5): 487 - 499.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R. Gonzalez-Ramos, J. Donnez, S. Defrere, I. Leclercq, J. Squifflet, J.-C. Lousse, and A. Van Langendonckt
Nuclear factor-kappa B is constitutively activated in peritoneal endometriosis
Mol. Hum. Reprod., July 1, 2007; 13(7): 503 - 509.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Arcuri, L. Buchwalder, P. Toti, M. Cintorino, P. Tosi, C. J. Lockwood, B. Rybalov, and F. Schatz
Differential Regulation of Colony Stimulating Factor 1 and Macrophage Migration Inhibitory Factor Expression by Inflammatory Cytokines in Term Human Decidua: Implications for Macrophage Trafficking at the Fetal-Maternal Interface
Biol Reprod, March 1, 2007; 76(3): 433 - 439.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. Ha, F. Hua, D. Grant, Y. Xia, J. Ma, X. Gao, J. Kelley, D. L. Williams, J. Kalbfleisch, I. W. Browder, et al.
Glucan phosphate attenuates cardiac dysfunction and inhibits cardiac MIF expression and apoptosis in septic mice
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1910 - H1918.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. H. Cook, J. Trgovcich, P. D. Zimmerman, Y. Zhang, and D. D. Sedmak
Lipopolysaccharide, Tumor Necrosis Factor Alpha, or Interleukin-1{beta} Triggers Reactivation of Latent Cytomegalovirus in Immunocompetent Mice.
J. Virol., September 1, 2006; 80(18): 9151 - 9158.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for the Study of Reproduction.