Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print June 1, 2005.
Biol Reprod 2005, 10.1095/biolreprod.105.040303
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BIOLOGY OF REPRODUCTION 73, 634–638 (2005)
DOI: 10.1095/biolreprod.105.040303
© 2005 by the Society for the Study of Reproduction, Inc.

LY6A/E (SCA-1) Expression in the Mouse Testis

Maaike P.A. van Bragt 1, 2, Nadia Ciliberti 3, William L. Stanford 3, Dirk G. de Rooij 2,4 , and Ans M.M. van Pelt 2

Department of Endocrinology,2 Faculty of Biology, Utrecht University, 3584 CH Utrecht, The Netherlands Institute of Biomaterials and Bioengineering,3 Department of Chemical Engineering and Applied Chemistry, Institute of Medical Science, University of Toronto, Toronto, Canada M5S 1A8 Department of Cell Biology,4 University Medical Center Utrecht, 3584 CH Utrecht, The Netherlands

Recently, it was found by two research groups that LY6A, known widely in the stem cell community as stem cell antigen-1 or SCA-1, is expressed on testicular side population (SP) cells. Whether these SP cells are spermatogonial stem cells is a point of disagreement and, therefore, the identity of the LY6A-positive cells as well. We studied the expression pattern of LY6A in testis by immunohistochemistry and found it to be expressed in the interstitial tissue on peritubular myoid, endothelial, and spherical-shaped peritubular mesenchymal cells. To address the question whether LY6A has a function in spermatogenesis or testis development, we studied the testis of Ly6a–/– mice (allele Ly6atm1Pmf). We found no morphological abnormalities or differences in numbers of spermatogonia, spermatocytes, Leydig cells, or macrophages in relation to the number of Sertoli cells. Therefore, we conclude that LY6A expression does not influence testis development or spermatogenesis and that spermatogonial stem cells are LY6A negative.

interstitial cells, Leydig cells, spermatogenesis, testis

1 Correspondence: Maaike van Bragt, Department of Endocrinology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. FAX: 31 030 253 2837; m.vanbragt{at}bio.uu.nl






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Copyright © 2005 by the Society for the Study of Reproduction.