Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print June 1, 2005.
Biol Reprod 2005, 10.1095/biolreprod.105.039701
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
73/4/648    most recent
biolreprod.105.039701v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Filby, A.L.
Right arrow Articles by Tyler, C.R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Filby, A.L.
Right arrow Articles by Tyler, C.R.
Agricola
Right arrow Articles by Filby, A.L.
Right arrow Articles by Tyler, C.R.
BIOLOGY OF REPRODUCTION 73, 648–662 (2005)
DOI: 10.1095/biolreprod.105.039701
© 2005 by the Society for the Study of Reproduction, Inc.

Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)1

A.L. Filby 2 , and C.R. Tyler 

Environmental and Molecular Fish Biology Group, Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Exeter, Devon EX4 4PS, United Kingdom

There are two estrogen receptor (ER) subtypes in fish, Esr1 and Esr2 (formerly ER{alpha} and ERß), and in some species the Esr2 subtype has two forms, Esr2b (formerly ERß1) and Esr2a (formerly ERß2 or ER{gamma}). There is little information, however, on the different characteristics and functional significance of the two receptor subtypes in fish, and this is especially relevant for understanding the disruption of ER signaling by chemicals with estrogenic activity. In this study, the full-length cDNAs for esr1 (3167 base pairs [bp]) and esr2b (2318 bp), and a partial-length (267 bp) cDNA for esr2a, were cloned and characterized in fathead minnow (fhm; Pimephales promelas), and their patterns of expression established during development and in adults. Real-time polymerase chain reaction revealed some clear distinctions in the ontogenic and tissue expression of fhm esr1, esr2b, and esr2a, suggesting different functions for each ER subtype. Fhm ERs were expressed in brain, pituitary, liver, gonad, intestine, and gill of male and female fish, esr2b and esr2a were also expressed in muscle. Fhm esr1 and esr2b were expressed predominantly in the liver, whereas fhm esr2a was expressed predominantly in intestine and was lowest expressed in liver. Responses of the different hepatic ERs in male fathead minnow exposed to 100 ng estradiol/L differed, with a significant induction (5-fold) of fhm esr1 but no effect on esr2b or esr2a expression, suggesting different mechanisms of regulation for the different ERs. The detailed characterization of ERs in fathead minnow provides the foundation for understanding the molecular basis of estrogenic disruption in fish.

developmental expression, endocrine disruption, estrogen receptors, fathead minnow, real-time polymerase chain reaction, tissue expression

1 Support was provided to A.F. for a Ph.D. studentship from the British Biotechnology and Biosciences Research Council (BBSRC).

2 Correspondence: FAX: 44 1392-263700; a.l.filby{at}exeter.ac.uk




This article has been cited by other articles:


Home page
 EndocrinologyHome page
A. Cohen, M. Shmoish, L. Levi, U. Cheruti, B. Levavi-Sivan, and E. Lubzens
Alterations in Micro-Ribonucleic Acid Expression Profiles Reveal a Novel Pathway for Estrogen Regulation
Endocrinology, April 1, 2008; 149(4): 1687 - 1696.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. L Filby, R. v. Aerle, J. Duitman, and C. R Tyler
The Kisspeptin/Gonadotropin-Releasing Hormone Pathway and Molecular Signaling of Puberty in Fish
Biol Reprod, February 1, 2008; 78(2): 278 - 289.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
T. Sabo-Attwood, J. L Blum, K. J Kroll, V. Patel, D. Birkholz, N. J Szabo, S. Z Fisher, R. McKenna, M. Campbell-Thompson, and N. D Denslow
Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol
J. Mol. Endocrinol., October 1, 2007; 39(4): 223 - 237.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Fuentes, P. M. Guerreiro, T. Modesto, J. Rotllant, A. V. M. Canario, and D. M. Power
A PTH/PTHrP receptor antagonist blocks the hypercalcemic response to estradiol-17beta
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R956 - R960.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
E. M. Santos, V. L. Workman, G. C. Paull, A. L. Filby, K. J. W. Van Look, P. Kille, and C. R. Tyler
Molecular basis of sex and reproductive status in breeding zebrafish
Physiol Genomics, July 18, 2007; 30(2): 111 - 122.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
E. F Orlando, Y. Katsu, S. Miyagawa, and T. Iguchi
Cloning and differential expression of estrogen receptor and aromatase genes in the self-fertilizing hermaphrodite and male mangrove rivulus, Kryptolebias marmoratus.
J. Mol. Endocrinol., October 1, 2006; 37(2): 353 - 365.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
A. L Filby, K. L Thorpe, and C. R Tyler
Multiple molecular effect pathways of an environmental oestrogen in fish.
J. Mol. Endocrinol., August 1, 2006; 37(1): 121 - 134.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for the Study of Reproduction.