HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 73/6/1219    most recent biolreprod.105.041616v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shimizu, A. Articles by Yoshimura, Y. Search for Related Content PubMed PubMed Citation Articles by Shimizu, A. Articles by Yoshimura, Y. Agricola Articles by Shimizu, A. Articles by Yoshimura, Y.

Impairment of Decidualization in SRC-Deficient Mice¹

Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan

ABSTRACT

Many signaling events induced by ovarian steroid hormones, cytokines, and growth factors are involved in the process of decidualization of human and rodent endometrium. We have reported previously that tyrosine kinase activation of SRC functionally participates in decidualization of human endometrial stromal cells. To address its essential role in decidualization, we examined, using wild-type and Src knockout mice, whether the process of decidualization was impaired in the absence of SRC. Immunohistochemistry using an antibody specific for the active form of SRC revealed that the active SRC was expressed prominently in the decidualizing stromal cells of the pregnant wild-type mouse. Moreover, the active SRC was upregulated in the uterine horn with artificially stimulated decidual reaction. In comparison with wild-type and Src heterozygous mice, the uterus of Src null mice showed no apparent decidual response following artificial stimulation. Ovarian steroid-induced decidualization in vitro, as determined by morphological changes and expression of decidual/trophoblast prolactin-related protein and prostaglandin-endoperoxide synthase 2 (also known as Cox2), both of which are decidualization markers, did not occur in a timely fashion in endometrial stromal cells isolated from the uteri of SRC-deficient mice compared to those from wild-type and Src heterozygous mice. Our results collectively suggest that SRC is an indispensable signaling component for maximal decidualization in mice.

decidua, estradiol, female reproductive tract, kinases, progesterone

¹ Supported, in part, by Grant-in-Aids for Young Scientists (13770938 to A.S.) and for Scientific Research (B15390511 to T.M. and B12470348 to Y.Y.) from the Japan Society for the Promotion of Science, by Keio Gijuku Academic Development Funds (to T.M.), and by grants from the Keio Health Counseling Center (to T.M.). Presented in part at the 11th World Congress of Gynecological Endocrinology, Florence, Italy, September 27–30, 2004, and at the ENDO 2005 meeting, San Diego, CA, June 4–7, 2005.

² Correspondence: Tetsuo Maruyama, Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. FAX: 81 3 3226 1667; tetsuo{at}sc.itc.keio.ac.jp

This article has been cited by other articles:

				T. Nagashima, T. Maruyama, H. Uchida, T. Kajitani, T. Arase, M. Ono, H. Oda, M. Kagami, H. Masuda, S. Nishikawa, et al. Activation of SRC Kinase and Phosphorylation of Signal Transducer and Activator of Transcription-5 Are Required for Decidual Transformation of Human Endometrial Stromal Cells Endocrinology, March 1, 2008; 149(3): 1227 - 1234. [Abstract] [Full Text] [PDF]

				I. Ihnatovych, W. Hu, J. L. Martin, A. T. Fazleabas, P. de Lanerolle, and Z. Strakova Increased Phosphorylation of Myosin Light Chain Prevents in Vitro Decidualization Endocrinology, July 1, 2007; 148(7): 3176 - 3184. [Abstract] [Full Text] [PDF]