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This Article Full Text Full Text (PDF) All Versions of this Article: 74/1/75    most recent biolreprod.105.044545v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moreau, C. Articles by Dupont, J. Search for Related Content PubMed PubMed Citation Articles by Moreau, C. Articles by Dupont, J. Agricola Articles by Moreau, C. Articles by Dupont, J.

Expression and Regulation of the SCD2 Desaturase in the Rat Ovary

Unité de Physiologie de la Reproduction et des Comportements,² Institut National de la Recherche Agronomique, 37380 Nouzilly, France Laboratory of Molecular Cancer Biology,³ 9052 Ghent, Belgium

ABSTRACT

Despite the significant role of the lipid reserve in cell structure and function, very few studies have provided detailed descriptions of unsaturated fatty acid synthesis in the ovary. In the present study, we have shown by RT-PCR, Northern blot, and Western blot analyses the mRNA and protein expression of SCD2 (stearoyl-coenzyme A desaturase 2; also named delta 9 desaturase) in rat ovary. We also have localized Scd2 mRNA by in situ hybridization, mainly in granulosa cells of antral follicles, cumulus oophorus, and corpus luteum. Interestingly, either no or very weak SCD2 expression was observed in primordial follicles and oocytes. After eCG injection for 24 h in immature rats (age, 22 days), the level of SCD2 expression and SCD activity in ovary was increased by approximately fourfold (P < 0.05), and the response was further increased 48 h after hCG treatment. As expected, eCG/hCG treatment increased expression of the steroidogenesis enzymes (CYP11A1 and HSD3B) and STAR. We also found a decrease in the SCD2 expression and SCD activity in the corpus luteum at Days 10 and 15 compared to Day 3 of gestation, paralleled by a decrease in the expression of the steroidogenesis enzymes and STAR. To investigate the molecular mechanisms involved in the regulation of SCD2 expression in ovary, we performed primary culture of rat granulosa cells. We observed that both insulin-like growth factor 1 (IGF1) (7.5 x 10^–8g/ml) and FSH (350 x 10^–8g/ml) increased SCD2 expression and SCD activity by approximately threefold. Using specific inhibitors, we demonstrated that the MAPK3/MAP1 and PIK3R1/AKT pathways are involved in the IGF1- and FSH-induced SCD2 expression, respectively. The SCD2 is expressed and active in rat ovary, and it may be involved in the regulation of follicular growth and/or the oocyte maturation.

follicle, follicle-stimulating hormone, granulosa cells, insulin-like growth factor receptor, ovary

¹ Correspondence: Joëlle Dupont, Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, Nouzilly 37 380, France. FAX: 33 2 47 42 77 43; jdupont{at}tours.inra.fr

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