| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
Prenatal Diagnosis Center,3 Cathay General Hospital, Taipei 106, Taiwan
School of Medicine,4 Fu Jen Catholic University, Hsinchung 242, Taiwan
College of Medicine,5 Taipei Medical University, Taipei 110, Taiwan
Bioresource Collection and Research Center,6 Food Industry Research and Development Institute, Hsinchu 300, Taiwan
Department of Medical Research,7 Taichung Veterans General Hospital, Taichung 407, Taiwan
ABSTRACT
Recent evidence has shown that amniotic fluid may be a novel source of fetal stem cells for therapeutic transplantation. We previously developed a two-stage culture protocol to isolate a population of amniotic fluid-derived mesenchymal stem cells (AFMSCs) from second-trimester amniocentesis. AFMSCs maintain the capacity to differentiate into multiple mesenchymal lineages and neuron-like cells. It is unclear whether amniotic fluid contains heterogeneous populations of stem cells or a subpopulation of primitive stem cells that are similar to marrow stromal cells showing the behavior of neural progenitors. In this study, we showed a subpopulation of amniotic fluid-derived stem cells (AF-SCs) at the single-cell level by limiting dilution. We found that NANOG- and POU5F1 (also known as OCT4)-expressing cells still existed in the expanded single cell-derived AF-SCs. Aside from the common mesenchymal characteristics, these clonal AF-SCs also exhibit multiple phenotypes of neural-derived cells such as NES, TUBB3, NEFH, NEUNA60, GALC, and GFAP expressions both before and after neural induction. Most importantly, HPLC analysis showed the evidence of dopamine release in the extract of dopaminergic-induced clonal AF-SCs. The results of this study suggest that besides being an easily accessible and expandable source of fetal stem cells, amniotic fluid will provide a promising source of neural progenitor cells that may be used in future cellular therapies for neurodegenerative diseases and nervous system injuries.
amniotic fluid cells, amniotic fluid-derived stem cells, developmental biology, dopamine, dopamine release, mesenchymal stem cells, neural stem cells, pregnancy
2 Correspondence: Ming-Song Tsai, Prenatal Diagnosis Center, Department of OBS/GYN, Cathay General Hospital, 280 Jen-Ai Road, Section 4, Taipei, 106, Taiwan. FAX: 886 2 23259530; mstsai{at}cgh.org.tw
1 Supported by grants from the National Science Council, Taiwan (NSC 92–2314-B-281–007 and NSC 93–2314-B-281–004) and the Ministry of Economic Affairs, Taiwan (93-EC-17-A-17-R7–0525).
This article has been cited by other articles:
|
|
 |
|
  W.-C. Lo, C.-H. Hsu, A. T.H. Wu, L.-Y. Yang, W.-H. Chen, W.-T. Chiu, W.-F. Lai, C.-H. Wu, J. G. Gelovani, and W.-P. Deng A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging J. Nucl. Med., September 1, 2008; 49(9): 1512 - 1519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. M. Extavour Evolution of the bilaterian germ line: lineage origin and modulation of specification mechanisms Integr. Comp. Biol., November 1, 2007; 47(5): 770 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-S. Tsai, S.-M. Hwang, K.-D. Chen, Y.-S. Lee, L.-W. Hsu, Y.-J. Chang, C.-N. Wang, H.-H. Peng, Y.-L. Chang, A.-S. Chao, et al. Functional Network Analysis of the Transcriptomes of Mesenchymal Stem Cells Derived from Amniotic Fluid, Amniotic Membrane, Cord Blood, and Bone Marrow Stem Cells, October 1, 2007; 25(10): 2511 - 2523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ilancheran, A. Michalska, G. Peh, E. M Wallace, M. Pera, and U. Manuelpillai Stem Cells Derived from Human Fetal Membranes Display Multilineage Differentiation Potential Biol Reprod, September 1, 2007; 77(3): 577 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Gargett Review Article: Stem Cells in Human Reproduction Reproductive Sciences, July 1, 2007; 14(5): 405 - 424. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Tateishi, E. Ashihara, N. Takehara, T. Nomura, S. Honsho, T. Nakagami, S. Morikawa, T. Takahashi, T. Ueyama, H. Matsubara, et al. Clonally amplified cardiac stem cells are regulated by Sca-1 signaling for efficient cardiovascular regeneration J. Cell Sci., May 15, 2007; 120(10): 1791 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Nagy, B. Gyorffy, O. Galamb, B. Molnar, B. Nagy, and Z. Papp Use of Routinely Collected Amniotic Fluid for Whole-Genome Expression Analysis of Polygenic Disorders Clin. Chem., November 1, 2006; 52(11): 2013 - 2020. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
