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BOR - Papers in Press, published online ahead of print February 1, 2006.
Biol Reprod 2006, 10.1095/biolreprod.105.047886
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BIOLOGY OF REPRODUCTION 74, 905–912 (2006)
DOI: 10.1095/biolreprod.105.047886
© 2006 by the Society for the Study of Reproduction, Inc.

Research Article

Neither Aurora B Activity nor Histone H3 Phosphorylation Is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes1

Lucie Jelínková , and Michal Kubelka 2 

Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 277 21 Libechov, Czech Republic

ABSTRACT

Aurora kinase B (AURKB) is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we examine expression and phosphorylation on Thr-232 of AURKB during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, whereas Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 µM butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10 µg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germinal vesicle stage, when nuclear membrane remains intact; however, condensed chromosome fibers or highly condensed chromosome bivalents can be seen in the nucleoplasm of BL-I- or cycloheximide-treated oocytes, respectively. In these treated oocytes, no or only very weak AURKB activity and phosphorylation of histone H3 on Ser-10 can be detected after 27 h of treatment, whereas phosphorylation on Ser-28 is not influenced. These results suggest that AURKB activity and Ser-10 phosphorylation of histone H3 are not required for chromosome condensation in pig oocytes, but might be required for further processing of chromosomes during meiosis.

gamete biology, kinases, meiosis, oocyte development

FOOTNOTES

1 Supported by the Institutional Research Plan of the Institute of Animal Physiology & Genetics no. AVOZ 50450515 and by grant no. 204/03/0816 of Grant Agency of the Czech Republic.

2 Correspondence: Michal Kubelka, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, 277 21 Libechov, Czech Republic. FAX: 420 315 639510; kubelka{at}iapg.cas.cz






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Copyright © 2006 by the Society for the Study of Reproduction.