HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 75/2/176    most recent biolreprod.106.052852v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hastings, J. M. Articles by Fazleabas, A. T. Search for Related Content PubMed PubMed Citation Articles by Hastings, J. M. Articles by Fazleabas, A. T. Agricola Articles by Hastings, J. M. Articles by Fazleabas, A. T.

The Estrogen Early Response Gene FOS Is Altered in a Baboon Model of Endometriosis¹

Department of Obstetrics and Gynecology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, 60612

ABSTRACT

Endometriosis, the presence of a functional endometrium outside of the uterine cavity, is associated with infertility. In our simulated model of pregnancy in baboons with experimental endometriosis, hCG infusion fails to induce expression of the immunoregulatory protein glycodelin. To test the hypothesis that the development of endometriosis is associated with an aberrant endometrial immunological environment, we examined the expression of a series of immunoregulatory genes in endometrium from baboons with and without endometriosis. Six months following intraperitoneal inoculation with menstrual endometrium, eutopic endometrium was surgically collected between Days 9 and 11 postovulation. Control endometrium was similarly collected from disease-free animals. Total RNA was extracted, and biotinylated cDNA probes were hybridized to the SuperArray GEArray Q series Th1/Th2/Th3 cDNA array, representing 96 genes. Gene expression levels were determined using ScanAlyze and GEArray Analyzer software. Seven genes were upregulated, including JUND, FOS, CCL11, NFKB1 and others, in the endometrium from baboons with endometriosis compared with the endometrium from disease-free animals; one gene, IL1R1, was downregulated. Quantitative RT-PCR confirmed upregulation of FOS and CCL11 in endometriotic eutopic endometrium. Immunohistochemical analysis revealed altered levels and distribution of FOS protein in the eutopic endometrium of baboons with induced endometriosis. These data suggest that in an induced model of endometriosis an aberrant eutopic immunological environment results in a decreased apoptotic potential and in rapid alterations in endometrial gene expression. We propose that the reduced fecundity associated with endometriosis has a multifold etiology in spontaneous and induced disease.

cytokines, gene regulation, immunology, steroid hormone receptors, uterus

¹ Supported by award U54 HD40093 from the National Institute of Child Health and Human Development Specialized Cooperative Centers Program in Research in Reproduction to A.T.F. and by a National Institutes of Health minority supplement to K.S.J.

² Correspondence: Asgerally T. Fazleabas, Department of Obstetrics and Gynecology, College of Medicine, University of Illinois at Chicago, MC808, 820 S Wood Street, Chicago, IL 60612. FAX: 312 996 8329; asgi{at}uic.edu

This article has been cited by other articles:

				J. D. Wren, Y. Wu, and S.-W. Guo A system-wide analysis of differentially expressed genes in ectopic and eutopic endometrium Hum. Reprod., August 1, 2007; 22(8): 2093 - 2102. [Abstract] [Full Text] [PDF]

				X. Zhang, C. Zhu, H. Lin, Q. Yang, Q. Ou, Y. Li, Z. Chen, P. Racey, S. Zhang, and H. Wang Wild Fulvous Fruit Bats (Rousettus leschenaulti) Exhibit Human-Like Menstrual Cycle Biol Reprod, August 1, 2007; 77(2): 358 - 364. [Abstract] [Full Text] [PDF]

				J.J. Kim, H.S. Taylor, Z. Lu, O. Ladhani, J.M. Hastings, K.S. Jackson, Y. Wu, S.W. Guo, and A.T. Fazleabas Altered expression of HOXA10 in endometriosis: potential role in decidualization Mol. Hum. Reprod., May 1, 2007; 13(5): 323 - 332. [Abstract] [Full Text] [PDF]

				C.J.P. Jones, J. Denton, and A.T. Fazleabas Morphological and glycosylation changes associated with the endometrium and ectopic lesions in a baboon model of endometriosis Hum. Reprod., December 1, 2006; 21(12): 3068 - 3080. [Abstract] [Full Text] [PDF]